Campàs Mònica, Prieto-Simón Beatriz, Marty Jean-Louis
IRTA, Ctra. Poble Nou, km. 5.5, 43540 Sant Carles de la Ràpita, Tarragona, Spain.
Semin Cell Dev Biol. 2009 Feb;20(1):3-9. doi: 10.1016/j.semcdb.2009.01.009. Epub 2009 Feb 4.
This article gives an overview of the electrochemical biosensors that incorporate genetically modified enzymes. Firstly, the improvements on the sensitivity and selectivity of biosensors that integrate mutated enzymes are summarised. Next, new trends focused on the oriented immobilisation of mutated enzymes through specific functional groups located at their surface are reviewed. Finally, the effect of enzyme mutations on the electron transfer distance and kinetics of electrochemical biosensors is described.
本文概述了包含基因修饰酶的电化学生物传感器。首先，总结了整合突变酶的生物传感器在灵敏度和选择性方面的改进。其次，综述了通过位于突变酶表面的特定官能团实现突变酶定向固定化的新趋势。最后，描述了酶突变对电化学生物传感器电子转移距离和动力学的影响。
