Žaloudíková Barbora, Kelbl Martin, Paša Libor, Freiberger Tomáš
Molecular Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic.
Department of Traumatology, Faculty of Medicine, Masaryk University, Trauma Hospital of Brno, Brno, Czech Republic.
J Med Microbiol. 2009 Jun;58(Pt 6):829-831. doi: 10.1099/jmm.0.006106-0.
A rare case of a severe prosthetic joint infection in a 71-year-old immunocompetent woman is presented. Listeria monocytogenes was identified in two consecutive samples using broad-range PCR and sequencing, whereas cultivation remained negative for the first sample and streptococci of a non-group A streptococci, non-group B streptococci type were detected for the second one. This report demonstrates that the phenotypic approach may lead to misidentification of L. monocytogenes in a routine clinical setting. Molecular methods of pathogen detection might be useful when a rare and/or unexpected micro-organism is present or the sample is collected during antibiotic treatment.
本文报告了一例71岁免疫功能正常女性发生的罕见严重人工关节感染病例。使用广谱PCR和测序技术在连续两份样本中鉴定出单核细胞增生李斯特菌，而第一份样本培养结果为阴性，第二份样本检测到非A群、非B群链球菌。本报告表明，在常规临床环境中，表型方法可能导致单核细胞增生李斯特菌的错误鉴定。当存在罕见和/或意外微生物或在抗生素治疗期间采集样本时，病原体检测的分子方法可能有用。
