血清细胞因子的顺序变化反映了小鼠柯萨奇病毒 B 感染靶器官中病毒 RNA 的动力学。
Sequential changes in serum cytokines reflect viral RNA kinetics in target organs of a coxsackievirus B infection in mice.
机构信息
Toxicology Division, National Food Administration, 752 27 Uppsala, Sweden.
出版信息
J Clin Immunol. 2009 Sep;29(5):611-9. doi: 10.1007/s10875-009-9294-8. Epub 2009 May 9.
INTRODUCTION
The pattern of cytokine responses related to viral replication during the course of the common human coxsackievirus B3 (CVB3) infection is not known.
METHODS
Serum levels of 21 cytokines and chemokines were studied (Luminex technique) in CVB3-infected in mice on days 3, 6, and 9 post-infection (p.i.). CVB3 was measured quantitatively (reverse transcriptase polymerase chain reaction) in the liver and pancreas.
RESULTS
Virus levels peaked on day 3 in both the liver and pancreas, but were 1,000-fold higher in the pancreas. IL-17alpha, IFN-gamma, KC, MCP-1, MIP1beta, and RANTES were detected on all days. On day 3 p.i., IL-6, IL-12(p40), KC, MCP-1, RANTES, and TNF-alpha were found to peak. On day 6 p.i., IL-1beta, IL-9, IL-12(p70), IL-13, IL-17alpha, and IFN-gamma peaked. On day 9 p.i., MIP1beta, IL-1beta, MCP-1, and TNF-alpha were still increased. These changes in cytokines may be used to monitor the progress of enteroviral infections in clinical settings.
简介
柯萨奇病毒 B3（CVB3）感染过程中与病毒复制相关的细胞因子反应模式尚不清楚。
方法
在感染后的第 3、6 和 9 天（感染后），使用 Luminex 技术研究了 21 种细胞因子和趋化因子在 CVB3 感染小鼠血清中的水平。使用逆转录聚合酶链反应（RT-PCR）定量检测肝脏和胰腺中的 CVB3。
结果
病毒水平在肝脏和胰腺中均于第 3 天达到峰值，但胰腺中的病毒水平高 1000 倍。在所有时间点均检测到 IL-17alpha、IFN-gamma、KC、MCP-1、MIP1beta 和 RANTES。在感染后第 3 天，IL-6、IL-12(p40)、KC、MCP-1、RANTES 和 TNF-alpha 达到峰值。在感染后第 6 天，IL-1beta、IL-9、IL-12(p70)、IL-13、IL-17alpha 和 IFN-gamma 达到峰值。在感染后第 9 天，MIP1beta、IL-1beta、MCP-1 和 TNF-alpha 仍在增加。这些细胞因子的变化可用于监测临床环境中肠道病毒感染的进展。
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