College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
Exp Mol Pathol. 2013 Feb;94(1):174-81. doi: 10.1016/j.yexmp.2012.06.006. Epub 2012 Jul 4.
To determine the relationship between viral kinetics and the expression patterns for different cytokines and chemokines in the serum and organs of coxsackievirus B3 (SSM-CVB3)-infected macaques over the course of infection.
SSM-CVB3 levels in serum and organs were measured using the Spearman-Karber 50% tissue culture infectious dose (TCID(50)) method. Cytokine and chemokine levels in the serum and organs were measured by indirect-ELISA.
Low viral titers were detected in the serum samples on the first day post-inoculation (p.i.) and peaked at 6 to 10 days p.i. in the serum samples from five macaques. Serum levels of IL-1β, IL-2, IL-6, IL-12p40, IL-17α, IFN-γ, TNF-α, MCP-1 and MIP-1β were detected each day and, similar to the viral titers, peaked at 6 to 10 days. IL-10 was only detected on days 10 to 14 p.i. Additionally, higher viral titers and relative viral mRNA levels were associated with higher cytokine and chemokine levels in selected tissues from infected macaques including heart, liver, spleen, lung, kidney and brain.
The results indicate that patterns of cytokine and chemokine response are associated with viral kinetics in the serum and target organs of SSM-CVB3-infected macaques, suggesting that the changes in cytokines and chemokines could help further our understanding of the progress of CVB3 infections in clinical settings.
确定柯萨奇病毒B3（SSM-CVB3）感染猕猴的过程中，病毒动力学与血清及各器官中不同细胞因子和趋化因子表达模式之间的关系。
采用Spearman-Karber 50%组织培养感染剂量（TCID(50)）法检测血清和器官中的SSM-CVB3水平。通过间接ELISA法检测血清和器官中的细胞因子和趋化因子水平。
接种后第1天在血清样本中检测到低病毒滴度，五只猕猴血清样本中的病毒滴度在接种后6至10天达到峰值。每天检测血清中IL-1β、IL-2、IL-6、IL-12p40、IL-17α、IFN-γ、TNF-α、MCP-1和MIP-1β的水平，与病毒滴度相似，在6至10天达到峰值。IL-10仅在接种后第10至14天检测到。此外，在感染猕猴的包括心脏、肝脏、脾脏、肺、肾脏和大脑在内的选定组织中，较高的病毒滴度和相对病毒mRNA水平与较高的细胞因子和趋化因子水平相关。
结果表明，细胞因子和趋化因子反应模式与SSM-CVB3感染猕猴的血清和靶器官中的病毒动力学相关，提示细胞因子和趋化因子的变化有助于我们进一步了解临床环境中CVB3感染的进展情况。
