Shinkai Takumi, Ohji Ryohei, Matsumoto Nobuya, Kobayashi Yasuo
FEMS Microbiol Lett. 2009 May;294(2):183-190. doi: 10.1111/j.1574-6968.2009.01565.x.
To characterize the fibrolytic function of Fibrobacter succinogenes strains in relation to their phylogenetic grouping, 32 strains were newly isolated from the rumen of sheep. All new strains were classified into phylogenetic groups 1 or 2 including a novel subgroup of group 2. Importantly, the majority of the strains belonging to group 1 were isolated from ruminally incubated hay. Although almost complete degradation of Avicel was observed among all strains, significantly lower digestibility of three different forages was recorded for strain HM2 of group 3 than for the strains of groups 1 and 2. In a comparison of all strains, two group 1 strains showed significantly higher digestibility of alfalfa and orchard grass hays, while two strains of the novel subgroup of group 2 had lower digestibility of orchard grass hay. Adhesion ability of each strain did not necessarily associate with the extent of digestibility. Maximum growth on Avicel was higher in group 1 than in group 2 strains, and two group 1 strains showed a shorter lag time. The results suggest that the ecological prominence of group 1 is due to a mixture of strains that are diverse in their fibrolytic capability making this group highly adaptable to any forage.
为了表征琥珀酸纤维杆菌菌株的纤维分解功能与其系统发育分组的关系，从绵羊瘤胃中新分离出32株菌株。所有新菌株被归类为系统发育组1或2，包括组2的一个新亚组。重要的是，属于组1的大多数菌株是从瘤胃培养的干草中分离出来的。虽然在所有菌株中都观察到了微晶纤维素的几乎完全降解，但与组1和组2的菌株相比，组3的HM2菌株对三种不同草料的消化率显著较低。在所有菌株的比较中，两株组1菌株对苜蓿和果园草干草的消化率显著较高，而组2新亚组的两株菌株对果园草干草的消化率较低。各菌株的黏附能力不一定与消化程度相关。组1菌株在微晶纤维素上的最大生长量高于组2菌株，且两株组1菌株的延迟期较短。结果表明，组1的生态优势归因于一组纤维分解能力不同的菌株，这使得该组能够高度适应任何草料。
