Biophys J. 1985 Aug;48(2):327-9. doi: 10.1016/S0006-3495(85)83786-3.
Melittin, a peptide from bee venom, is known to undergo a monomer / tetramer conversion in aqueous solutions. We have studied the possible participation of dimers in the association equilibrium of melittin by sedimentation equilibrium experiments in the analytical ultracentrifuge and subsequent mathematical analysis of the concentration distributions obtained. It was found that the dimeric state is not significantly populated, the contribution of dimer to the total peptide weight probably being below 0.5%.
蜂毒肽是一种来自蜜蜂毒液的肽,已知它在水溶液中会发生单体/四聚体转化。我们通过分析超速离心的沉降平衡实验以及随后对获得的浓度分布进行的数学分析,研究了二聚体在蜂毒肽缔合平衡中可能的参与。结果发现二聚体状态的丰度并不显著,二聚体对总肽重量的贡献可能低于 0.5%。