Biophys J. 1987 Jan;51(1):145-8. doi: 10.1016/S0006-3495(87)83319-2.
Simultaneously measured low temperature absorption and circular dichroic spectra are presented for different intermediates of the bacteriorhodopsin photocycle in suspension and hydrated film of purple membranes. The data for the L intermediate are in accord with excitonic interpretation of the visible part of the circular dichroic spectrum, suggesting that no large scale structural change of the purple membrane affecting its crystalline structure happens during the L formation. The structure of the membrane, which is disrupted in the M state, is recovered when M is illuminated with blue light at low temperature.
同时测量了菌紫质光循环不同中间产物在悬浮和紫膜水合膜中的低温吸收和圆二色光谱。L 中间产物的数据与圆二色光谱可见部分的激子解释相符,表明在 L 形成过程中紫膜的结晶结构没有发生大的结构变化。在 M 态时,膜的结构被破坏,当用蓝光低温照射 M 时,膜的结构得到恢复。