Xue Zhaohui, Yu Wancong, Liu Zhiwei, Wu Moucheng, Kou Xiaohong, Wang Jiehua
School of Agriculture and Bioengineering, Tianjin University, Tianjin, Peoples' Republic of China.
J Agric Food Chem. 2009 Jun 24;57(12):5287-93. doi: 10.1021/jf900860v.
This study investigated the possibility of converting the insoluble rapeseed meal protein into functionally active ingredients for food applications. The rapeseed ( Brassica napus ) meal protein isolates were first digested by Alcalase and Flavourzyme, and the resultant rapeseed crude hydrolysate (RSCH) exhibited a dose-dependent reducing antioxidant power and hydroxyl radical scavenging ability. RSCH could also inhibit the malonyldialdehyde (MDA) generation by 50% in blood serum at 150 mg/mL. RSCH was further separated into three fractions (RSP1, RSP2, and RSP3) by Sephadex gel filtration according to their different molecular weights. The amino acid compositions and antioxidant potentials were assessed for RSP1-3 fractions. All three fractions showed inhibiting effects on superoxide anion generation to various extents. They could also inhibit the autohemolysis of rat red blood cells and MDA formation in rat liver tissue homogenate. The results suggested that rapeseed peptide hydrolysate may be useful as a human food addition as a source of bioactive peptides with antioxidant properties.
本研究探讨了将不溶性菜籽粕蛋白转化为具有功能活性的食品应用成分的可能性。首先用碱性蛋白酶和风味酶消化菜籽（甘蓝型油菜）粕分离蛋白，所得菜籽粗水解物（RSCH）表现出剂量依赖性的还原抗氧化能力和羟基自由基清除能力。RSCH在浓度为150mg/mL时还能抑制血清中丙二醛（MDA）生成50%。通过葡聚糖凝胶过滤根据分子量不同将RSCH进一步分离为三个组分（RSP1、RSP2和RSP3）。对RSP1 - 3组分进行了氨基酸组成和抗氧化潜力评估。所有三个组分均对超氧阴离子生成有不同程度的抑制作用。它们还能抑制大鼠红细胞的自溶以及大鼠肝组织匀浆中MDA的形成。结果表明，菜籽肽水解物作为具有抗氧化特性的生物活性肽来源，可能用作人类食品添加剂。
