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[医学实验室生物样本质量评估:以科特迪瓦巴斯德研究所为例]

[Assessment of biological sampling's quality in a medical laboratory: case of Côte d' Ivoire Institute Pasteur].

作者信息

Kouassi-M'Bengue Alphonsine, Koffi Stephane, Manizan Pascale, Ouattara Abdoulaye, N'Douba Adele Kacou, Dosso Mireille

机构信息

UFR des Sciences Médicales d' Abidjan Département de Microbiologie, Institut Pasteur de Côte d'Ivoire Laboratoire de Bactériologie, Abidjan.

出版信息

Mali Med. 2008;23(2):16-20.

PMID:19434962
Abstract

BACKGROUND

Assurance quality is important in medical laboratory, but in Africa, few laboratories are involved in this process. The aim of this study was to assess biological sampling's quality in a bacteriological laboratory.

MATERIAL AND METHODS

A cross sectional study was undertaken in medical bacteriological laboratory of Côte d' Ivoire Institute Pasteur during 6 months. All urines, saddles, and bronchial expectorations collected from ambulatory patients during this period were included in the study. The quality of urine's, saddles and bronchial expectorations' sampling for a bacteriological analysis was evaluated. An interview based on Guidelines of good laboratories practices and referential ISO 15189 was used. A total of 300 samples were indexed.

RESULTS

On a total of 300 recorded biological samples, 224 (74.7%) were not in conformity. In 87.5% of the cases of nonconformities, an antibiotic's treatment were preliminary instituted before the sampling. Corrective actions were carried in the laboratory on 30 samples with 56.6% for the urines, 26.7% for the saddles and 16.7% for the bronchial expectorations.

CONCLUSION

At the end of this study, it arises that the quality of the biological sampling received at the medical bacteriology laboratory need to be improved.

摘要

背景

质量保证在医学实验室中很重要,但在非洲,很少有实验室参与这一过程。本研究的目的是评估一家细菌学实验室中生物样本采集的质量。

材料与方法

在科特迪瓦巴斯德研究所的医学细菌学实验室进行了为期6个月的横断面研究。在此期间从门诊患者收集的所有尿液、粪便和支气管痰液都纳入了研究。对用于细菌学分析的尿液、粪便和支气管痰液样本采集的质量进行了评估。采用了基于良好实验室规范指南和ISO 15189标准的访谈。共对300个样本进行了编目。

结果

在总共300份记录的生物样本中,224份(74.7%)不符合要求。在87.5%的不符合案例中,在采样前就初步进行了抗生素治疗。实验室对30个样本采取了纠正措施,其中尿液样本占56.6%,粪便样本占26.7%,支气管痰液样本占16.7%。

结论

在本研究结束时发现,医学细菌学实验室接收的生物样本采集质量需要提高。

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