Wei Zhan-Yong, Wang Xue-Bin, Ning Xiao-Dong, Wang Ya-Bin, Zhang Hong-Ying, Wang Dong-Fang, Chen Hong-Ying, Cui Bao-An
The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Wenhua Road 95#, 450002, Zhengzhou, Henan, People's Republic of China.
Arch Virol. 2009;154(6):999-1003. doi: 10.1007/s00705-009-0392-y. Epub 2009 May 13.
This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-L-acetylpenicillamine (SNAP) and L-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-L-arginine methyl ester (L-NAME). By assaying the steps of the PPV life cycle, we also show that NO inhibits viral DNA and protein synthesis. This experiment provides a frame of reference for the study of the anti-viral mechanism of NO.
本研究探讨了一氧化氮（NO）对猪细小病毒（PPV）在PK - 15细胞中复制的抑制作用及其机制。结果表明，两种NO生成化合物，即S - 亚硝基 - L - 乙酰青霉胺（SNAP）和L - 精氨酸（LA），在非细胞毒性浓度下可呈剂量依赖性地降低PPV复制，且这种抗PPV效应可被一氧化氮合酶（NOS）抑制剂N - 硝基 - L - 精氨酸甲酯（L - NAME）逆转。通过检测PPV生命周期的各个步骤，我们还表明NO抑制病毒DNA和蛋白质合成。本实验为研究NO的抗病毒机制提供了参考框架。
