• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

产生一氧化氮的化合物GSNO在体外和体内均可抑制猪圆环病毒2型感染。

Nitric oxide-generating compound GSNO suppresses porcine circovirus type 2 infection in vitro and in vivo.

作者信息

Liu Chuanmin, Wen Libin, Xiao Qi, He Kongwang

机构信息

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, 50 Zhong-ling Street, Xuanwu District, Nanjing, 210014, China.

Key laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, 50 Zhong-ling Street, Xuanwu District, Nanjing, 210014, China.

出版信息

BMC Vet Res. 2017 Feb 21;13(1):59. doi: 10.1186/s12917-017-0976-9.

DOI:10.1186/s12917-017-0976-9
PMID:28222773
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5320642/
Abstract

BACKGROUND

Nitric oxide (NO), an important signaling molecule with biological functions, has antimicrobial activity against a variety of pathogens including viruses. To our knowledge, little information is available about the regulatory effect of NO on porcine circovirus type 2 (PCV2) infection. This study was conducted to investigate the antiviral activity of NO generated from S-nitrosoglutathione (GSNO), during PCV2 infection of PK-15 cells and BALB/c mice.

RESULTS

GSNO released considerable NO in the culture medium of PK-15 cells, and NO was scavenged by its scavenger hemoglobin (Hb) in a dose-dependent manner. NO strongly inhibited PCV2 replication in PK-15 cells, and the antiviral effect was reversed by Hb. An in vivo assay indicated that GSNO treatment reduced the progression of PCV2 infection in mice, evident as reductions in the percentages of PCV2-positive sera and tissue samples and in the viral DNA copies in serum samples. GSNO also improved the growth performance and immune organs (spleens and thymuses) of the PCV2-infected mice to some degree.

CONCLUSIONS

Our data demonstrate that the NO-generating compound GSNO suppresses PCV2 infection in PK-15 cells and BALB/c mice, indicating that NO and its donor, GSNO, have potential value as antiviral drugs against PCV2 infection.

摘要

背景

一氧化氮（NO）是一种具有生物学功能的重要信号分子，对包括病毒在内的多种病原体具有抗菌活性。据我们所知，关于NO对猪圆环病毒2型（PCV2）感染的调节作用的信息很少。本研究旨在探讨在PK-15细胞和BALB/c小鼠感染PCV2期间，由S-亚硝基谷胱甘肽（GSNO）产生的NO的抗病毒活性。

结果

GSNO在PK-15细胞培养基中释放出大量NO，且NO被其清除剂血红蛋白（Hb）以剂量依赖方式清除。NO强烈抑制PK-15细胞中PCV2的复制，且该抗病毒作用被Hb逆转。体内试验表明，GSNO处理可减轻小鼠PCV2感染的进展，表现为PCV2阳性血清和组织样本的百分比以及血清样本中病毒DNA拷贝数的减少。GSNO还在一定程度上改善了PCV2感染小鼠的生长性能和免疫器官（脾脏和胸腺）。

结论

我们的数据表明，产生NO的化合物GSNO可抑制PK-15细胞和BALB/c小鼠中的PCV2感染，表明NO及其供体GSNO作为抗PCV2感染的抗病毒药物具有潜在价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/19d0c3b4c72e/12917_2017_976_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/c07d5a9c945e/12917_2017_976_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/3448b5000238/12917_2017_976_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/373199aa07fd/12917_2017_976_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/54507c4bbd7a/12917_2017_976_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/677d3aaa9a2c/12917_2017_976_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/19d0c3b4c72e/12917_2017_976_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/c07d5a9c945e/12917_2017_976_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/3448b5000238/12917_2017_976_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/373199aa07fd/12917_2017_976_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/54507c4bbd7a/12917_2017_976_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/677d3aaa9a2c/12917_2017_976_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef08/5320642/19d0c3b4c72e/12917_2017_976_Fig6_HTML.jpg

相似文献

1
Nitric oxide-generating compound GSNO suppresses porcine circovirus type 2 infection in vitro and in vivo.产生一氧化氮的化合物GSNO在体外和体内均可抑制猪圆环病毒2型感染。
BMC Vet Res. 2017 Feb 21;13(1):59. doi: 10.1186/s12917-017-0976-9.
2
Exogenous nitric oxide generated from SNAP blocks porcine circovirus type 2 replication and regulates NF-κB activity in PK-15 cells.外源性一氧化氮通过 SNAP 阻断猪圆环病毒 2 复制，并调节 PK-15 细胞中 NF-κB 的活性。
Int J Antimicrob Agents. 2023 Aug;62(2):106859. doi: 10.1016/j.ijantimicag.2023.106859. Epub 2023 May 26.
3
Adenovirus-mediated shRNA interference against porcine circovirus type 2 replication both in vitro and in vivo.腺病毒介导的针对猪圆环病毒2型的短发夹RNA干扰在体外和体内均对其复制产生影响。
Antiviral Res. 2008 Mar;77(3):186-94. doi: 10.1016/j.antiviral.2007.11.005. Epub 2007 Dec 18.
4
Ivermectin treatment inhibits the replication of Porcine circovirus 2 (PCV2) in vitro and mitigates the impact of viral infection in piglets.伊维菌素治疗可抑制猪圆环病毒 2（PCV2）的体外复制，并减轻仔猪感染病毒的影响。
Virus Res. 2019 Apr 2;263:80-86. doi: 10.1016/j.virusres.2019.01.010. Epub 2019 Jan 15.
5
Effect of atovastatin treatment on porcine circovirus 2 infection in BALB/c mice.阿托伐他汀治疗对BALB/c小鼠猪圆环病毒2感染的影响。
Clin Exp Pharmacol Physiol. 2015 Aug;42(8):817-21. doi: 10.1111/1440-1681.12434.
6
The antiviral activity of arctigenin in traditional Chinese medicine on porcine circovirus type 2.中药牛蒡子苷元对猪圆环病毒2型的抗病毒活性
Res Vet Sci. 2016 Jun;106:159-64. doi: 10.1016/j.rvsc.2015.10.012. Epub 2015 Oct 26.
7
A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro.一种自由基形式的一氧化氮在体外抑制猪圆环病毒2型的复制。
BMC Vet Res. 2019 Feb 1;15(1):47. doi: 10.1186/s12917-019-1796-x.
8
The Hsp90 inhibitor 17-DMAG decreases infection of porcine circovirus type 2 in mice.热休克蛋白90抑制剂17-DMAG可降低小鼠2型猪圆环病毒感染率。
Microb Pathog. 2017 Aug;109:248-252. doi: 10.1016/j.micpath.2017.06.007. Epub 2017 Jun 7.
9
PDZK1 upregulates nitric oxide production through the PI3K/ERK2 pathway to inhibit porcine circovirus type 2 replication.PDZK1通过PI3K/ERK2途径上调一氧化氮的产生，以抑制猪圆环病毒2型的复制。
Vet Microbiol. 2022 Sep;272:109514. doi: 10.1016/j.vetmic.2022.109514. Epub 2022 Jul 19.
10
Hsp90 inhibitor reduces porcine circovirus 2 replication in the porcine monocytic line 3D4/31.热休克蛋白90抑制剂可降低猪圆环病毒2型在猪单核细胞系3D4/31中的复制。
Virus Genes. 2017 Feb;53(1):95-99. doi: 10.1007/s11262-016-1385-z. Epub 2016 Aug 24.

引用本文的文献

1
Nitric oxide and viral infection: Recent developments in antiviral therapies and platforms.一氧化氮与病毒感染：抗病毒疗法及平台的最新进展
Appl Mater Today. 2021 Mar;22:100887. doi: 10.1016/j.apmt.2020.100887. Epub 2020 Dec 5.
2
Saying no to SARS-CoV-2: the potential of nitric oxide in the treatment of COVID-19 pneumonia.拒绝 SARS-CoV-2：一氧化氮在治疗 COVID-19 肺炎中的潜力。
Med Gas Res. 2024 Apr-Jun;14(2):39-47. doi: 10.4103/2045-9912.385414.
3
Oral administration of S-nitroso-L-glutathione (GSNO) provides anti-inflammatory and cytoprotective effects during ocular bacterial infections.

本文引用的文献

1
Matrine displayed antiviral activity in porcine alveolar macrophages co-infected by porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.苦参碱在猪繁殖与呼吸综合征病毒和猪圆环病毒2型共同感染的猪肺泡巨噬细胞中表现出抗病毒活性。
Sci Rep. 2016 Apr 15;6:24401. doi: 10.1038/srep24401.
2
Porcine circovirus type 2 activates PI3K/Akt and p38 MAPK pathways to promote interleukin-10 production in macrophages via Cap interaction of gC1qR.猪圆环病毒2型通过gC1qR的Cap相互作用激活PI3K/Akt和p38 MAPK信号通路，以促进巨噬细胞中白细胞介素-10的产生。
Oncotarget. 2016 Apr 5;7(14):17492-507. doi: 10.18632/oncotarget.7362.
3
口服 S-亚硝基-L-谷胱甘肽（GSNO）可在眼部细菌感染期间提供抗炎和细胞保护作用。
Cell Mol Life Sci. 2023 Sep 28;80(10):309. doi: 10.1007/s00018-023-04963-w.
4
Roles and current applications of S-nitrosoglutathione in anti-infective biomaterials.S-亚硝基谷胱甘肽在抗感染生物材料中的作用及当前应用
Mater Today Bio. 2022 Sep 6;16:100419. doi: 10.1016/j.mtbio.2022.100419. eCollection 2022 Dec.
5
Pyroptotic Patterns in Blood Leukocytes Predict Disease Severity and Outcome in COVID-19 Patients.血液白细胞的 pyroptotic 模式可预测 COVID-19 患者的疾病严重程度和预后。
Front Immunol. 2022 Jul 19;13:888661. doi: 10.3389/fimmu.2022.888661. eCollection 2022.
6
NO in Viral Infections: Role and Development of Antiviral Therapies.病毒感染中的一氧化氮：抗病毒治疗的作用和发展。
Molecules. 2022 Apr 5;27(7):2337. doi: 10.3390/molecules27072337.
7
Nitric oxide (NO) and nanoparticles - Potential small tools for the war against COVID-19 and other human coronavirus infections.一氧化氮 (NO) 和纳米颗粒——抗击 COVID-19 和其他人类冠状病毒感染的潜在小工具。
Virus Res. 2021 Jan 2;291:198202. doi: 10.1016/j.virusres.2020.198202. Epub 2020 Oct 18.
8
Mouse models of porcine circovirus 2 infection.猪圆环病毒2型感染的小鼠模型
Animal Model Exp Med. 2018 Apr 19;1(1):23-28. doi: 10.1002/ame2.12009. eCollection 2018 Mar.
9
A radical form of nitric oxide inhibits porcine circovirus type 2 replication in vitro.一种自由基形式的一氧化氮在体外抑制猪圆环病毒2型的复制。
BMC Vet Res. 2019 Feb 1;15(1):47. doi: 10.1186/s12917-019-1796-x.
Spotlights on immunological effects of reactive nitrogen species: When inflammation says nitric oxide.
活性氮物质的免疫效应聚焦：当炎症遇上一氧化氮时
World J Exp Med. 2015 May 20;5(2):64-76. doi: 10.5493/wjem.v5.i2.64.
4
Nitric oxide synthases, S-nitrosylation and cardiovascular health: from molecular mechanisms to therapeutic opportunities (review).一氧化氮合酶、S-亚硝基化与心血管健康：从分子机制到治疗机遇（综述）
Mol Med Rep. 2015 Mar;11(3):1555-65. doi: 10.3892/mmr.2014.2968. Epub 2014 Nov 18.
5
Susceptibility of calves to porcine circovirus-2 (PCV2).犊牛对猪圆环病毒2型（PCV2）的易感性。
Vet Microbiol. 2014 Sep 17;173(1-2):125-31. doi: 10.1016/j.vetmic.2014.06.022. Epub 2014 Jul 17.
6
Can Porcine circovirus type 2 (PCV2) infection be eradicated by mass vaccination?猪圆环病毒2型（PCV2）感染能通过大规模疫苗接种根除吗？
Vet Microbiol. 2014 Aug 6;172(1-2):92-9. doi: 10.1016/j.vetmic.2014.05.003. Epub 2014 May 13.
7
Control of early Theiler's murine encephalomyelitis virus replication in macrophages by interleukin-6 occurs in conjunction with STAT1 activation and nitric oxide production.白细胞介素-6 通过激活 STAT1 和产生一氧化氮来控制巨噬细胞中早期脊髓灰质炎病毒的复制。
J Virol. 2012 Oct;86(19):10841-51. doi: 10.1128/JVI.01402-12. Epub 2012 Jul 25.
8
The potential of nitric oxide releasing therapies as antimicrobial agents.一氧化氮释放疗法作为抗菌剂的潜力。
Virulence. 2012 May 1;3(3):271-9. doi: 10.4161/viru.20328.
9
The nitric oxide pathway provides innate antiviral protection in conjunction with the type I interferon pathway in fibroblasts.一氧化氮通路与成纤维细胞中的 I 型干扰素通路一起提供先天抗病毒保护。
PLoS One. 2012;7(2):e31688. doi: 10.1371/journal.pone.0031688. Epub 2012 Feb 21.
10
IFN-γ production depends on IL-12 and IL-18 combined action and mediates host resistance to dengue virus infection in a nitric oxide-dependent manner.IFN-γ 的产生依赖于 IL-12 和 IL-18 的联合作用，并通过一氧化氮依赖的方式介导宿主对登革病毒感染的抵抗力。
PLoS Negl Trop Dis. 2011 Dec;5(12):e1449. doi: 10.1371/journal.pntd.0001449. Epub 2011 Dec 20.