反义寡核苷酸抑制葡萄糖转运蛋白-1 对人喉癌细胞增殖和葡萄糖摄取的作用。
Inhibition of cell proliferation and glucose uptake in human laryngeal carcinoma cells by antisense oligonucleotides against glucose transporter-1.
机构信息
Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
出版信息
Head Neck. 2009 Dec;31(12):1624-33. doi: 10.1002/hed.21137.
BACKGROUND
Malignant cells show increased glucose uptake in vitro and in vivo, which is thought to be mediated by glucose transporters. In this study, we investigated the effect of plasmid-derived antisense RNA against the Glut-l gene on proliferation and glucose uptake in laryngeal carcinoma Hep-2 cells.
METHODS
The expression plasmids pcDNA3.1(+)-Glut-1 and pcDNA3.1(+)-anti Glut-1 were constructed. The MTT method was used to assess cell growth inhibition. The expression of Glut-1 mRNA and protein was detected by reverse transcriptase-polymerase chain reaction and Western blotting, respectively.
RESULTS
After transfection, Glut-1 AS clearly inhibited glucose uptake and cell growth in Hep-2 cells, and we observed a decrease in the expression of Glut-1 mRNA and protein in Hep-2 cells.
CONCLUSIONS
Glut-1 AS decreases glucose uptake and inhibits the proliferation of Hep-2 cells.
背景
恶性细胞在体外和体内显示出葡萄糖摄取增加，这被认为是由葡萄糖转运蛋白介导的。在这项研究中，我们研究了针对 Glut-1 基因的质粒衍生反义 RNA 对喉癌细胞 Hep-2 增殖和葡萄糖摄取的影响。
方法
构建表达质粒 pcDNA3.1(+)-Glut-1 和 pcDNA3.1(+)-anti Glut-1。使用 MTT 法评估细胞生长抑制。通过逆转录-聚合酶链反应和 Western blot 分别检测 Glut-1 mRNA 和蛋白的表达。
结果
转染后，Glut-1 AS 明显抑制 Hep-2 细胞的葡萄糖摄取和细胞生长，我们观察到 Hep-2 细胞中 Glut-1 mRNA 和蛋白的表达下降。
结论
Glut-1 AS 减少葡萄糖摄取并抑制 Hep-2 细胞的增殖。
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