von Allmen J M, Rottmann W H, Gengenbach B G, Harvey A J, Lonsdale D M
Cambridge Laboratory, John Innes Centre, Norwich, UK.
Mol Gen Genet. 1991 Oct;229(3):405-12. doi: 10.1007/BF00267463.
The mitochondrial gene, T-urf13, which is unique to the T-cytoplasm of maize, has been expressed in tobacco plants using the Cauliflower Mosaic Virus 35S promoter. Tobacco plants expressing T-urf13 exhibit a variety of responses to methomyl. Leaf discs and petiole sections bleach when exposed to methomyl or HmT-toxin; this effect increases with the age of the tissue. The bleaching effect is not however observed when light is excluded. Plants homozygous for T-urf13 exhibit extreme sensitivity when sprayed with methomyl. The growth of seedling which are either homozygous or heterozygous for T-urf13 is inhibited by methomyl and by kanamycin, whereas seedlings from untransformed tobacco or tobacco which has lost the T-urf13 gene through segregation are sensitive to kanamycin but develop normally when exposed to methomyl. The results demonstrate that T-URF13 need not be specifically targeted to the mitochondrion for it to induce methomyl or HmT-toxin sensitivity in tobacco.
线粒体基因T-urf13是玉米T细胞质所特有的，已利用花椰菜花叶病毒35S启动子在烟草植株中进行了表达。表达T-urf13的烟草植株对灭多威表现出多种反应。叶盘和叶柄切片在接触灭多威或HmT毒素时会变白；这种效应随着组织年龄的增长而增强。然而，在排除光照时未观察到变白效应。T-urf13纯合的植株在喷洒灭多威时表现出极度敏感性。T-urf13纯合或杂合的幼苗生长受到灭多威和卡那霉素的抑制，而未转化烟草或通过分离失去T-urf13基因的烟草的幼苗对卡那霉素敏感，但在接触灭多威时正常生长。结果表明，T-URF13无需特异性定位于线粒体即可在烟草中诱导对灭多威或HmT毒素的敏感性。
