Alani R, Brown P, Binétruy B, Dosaka H, Rosenberg R K, Angel P, Karin M, Birrer M J
NCI-Navy Medical Oncology Branch, Bethesda, Maryland.
Mol Cell Biol. 1991 Dec;11(12):6286-95. doi: 10.1128/mcb.11.12.6286-6295.1991.
The nuclear phosphoprotein c-Jun, encoded by the proto-oncogene c-jun, is a major component of the AP-1 complex. A potent transcriptional regulator, c-jun is also able to transform normal rat embryo cells in cooperation with an activated c-Ha-ras gene. By deletion analysis, we identified the regions of c-Jun encoding transformation and transactivation functions. Our studies indicate that there is a direct correlation between the ability of the c-Jun protein to activate transcription and cotransform rat embryo cells. The regions involved in these functions include the conserved leucine zipper/DNA binding domain and an effector domain near its N terminus. This N-terminal region spans amino acids 61 to 146 of the c-Jun protein and is highly conserved among all Jun family members. These results support the hypothesis that c-Jun transforms cells by stimulating the expression of transformation-mediating genes.
原癌基因c-jun编码的核磷蛋白c-Jun是AP-1复合物的主要成分。作为一种有效的转录调节因子，c-Jun还能够与激活的c-Ha-ras基因协同作用，使正常大鼠胚胎细胞发生转化。通过缺失分析，我们确定了c-Jun编码转化和反式激活功能的区域。我们的研究表明，c-Jun蛋白激活转录的能力与共转化大鼠胚胎细胞的能力之间存在直接相关性。参与这些功能的区域包括保守的亮氨酸拉链/DNA结合结构域及其N端附近的效应结构域。这个N端区域跨越c-Jun蛋白的第61至146个氨基酸，在所有Jun家族成员中高度保守。这些结果支持了c-Jun通过刺激转化介导基因的表达来转化细胞的假说。
