Hsiao W L, Gattoni-Celli S, Weinstein I B
Science. 1984 Nov 2;226(4674):552-5. doi: 10.1126/science.6436974.
The tumor promoters 12-O-tetradecanoyl-phorbol-13-acetate and teleocidin markedly enhanced the transformation of C3H 10T1/2 mouse fibroblasts when these cells were transfected with the cloned human bladder cancer c-rasH oncogene. Transfection studies with the drug resistance marker gpt and time course studies indicate that this enhancement is not simply an effect on the process of DNA transfection. These findings, together with parallel studies with NIH 3T3 fibroblasts, also indicate that the competence of animal cells for DNA transfection is a function of the recipient cell line, the transfected marker, and the growth conditions. Our findings suggest that during multistage carcinogenesis tumor promoters may complement the function of activated cellular oncogenes.
当用克隆的人膀胱癌c-rasH癌基因转染C3H 10T1/2小鼠成纤维细胞时，肿瘤促进剂12-O-十四烷酰佛波醇-13-乙酸酯和远霉素A显著增强了这些细胞的转化。用耐药标记物gpt进行的转染研究和时间进程研究表明，这种增强不仅仅是对DNA转染过程的影响。这些发现，连同对NIH 3T3成纤维细胞的平行研究，也表明动物细胞进行DNA转染的能力是受体细胞系、转染标记物和生长条件的函数。我们的发现表明，在多阶段致癌过程中，肿瘤促进剂可能补充活化的细胞癌基因的功能。
