Vidal M, Strich R, Esposito R E, Gaber R F
Department of Biochemistry, Molecular Biology, Northwestern University, Evanston, Illinois 60208-3500.
Mol Cell Biol. 1991 Dec;11(12):6306-16. doi: 10.1128/mcb.11.12.6306-6316.1991.
We show that the extent of transcriptional regulation of many, apparently unrelated, genes in Saccharomyces cerevisiae is dependent on RPD1 (and RPD3 [M. Vidal and R. F. Gaber, Mol. Cell. Biol. 11:6317-6327, 1991]). Genes regulated by stimuli as diverse as external signals (PHO5), cell differentiation processes (SPO11 and SPO13), cell type (RME1, FUS1, HO, TY2, STE6, STE3, and BAR1), and genes whose regulatory signals remain unknown (TRK2) depend on RPD1 to achieve maximal states of transcriptional regulation. RPD1 enhances both positive and negative regulation of these genes: in rpd1 delta mutants, higher levels of expression are observed under repression conditions and lower levels are observed under activation conditions. We show that several independent genetic screens, designed to identify yeast transcriptional regulators, have detected the RPD1 locus (also known as SIN3, SD11, and UME4). The inferred RPD1 protein contains four regions predicted to take on helix-loop-helix-like secondary structures and three regions (acidic, glutamine rich, and proline rich) reminiscent of the activating domains of transcriptional activators.
我们发现，酿酒酵母中许多明显不相关的基因的转录调控程度取决于RPD1（以及RPD3 [M. Vidal和R. F. Gaber，《分子与细胞生物学》11:6317 - 6327，1991年]）。受多种刺激调控的基因，如外部信号（PHO5）、细胞分化过程（SPO11和SPO13）、细胞类型（RME1、FUS1、HO、TY2、STE6、STE3和BAR1），以及调控信号未知的基因（TRK2），都依赖RPD1来实现转录调控的最大状态。RPD1增强了这些基因的正向和负向调控：在rpd1δ突变体中，在抑制条件下观察到更高的表达水平，在激活条件下观察到更低的表达水平。我们表明，旨在鉴定酵母转录调节因子的几个独立遗传筛选都检测到了RPD1基因座（也称为SIN3、SD11和UME4）。推断的RPD1蛋白包含四个预计具有螺旋 - 环 - 螺旋样二级结构的区域，以及三个让人联想到转录激活因子激活域的区域（酸性、富含谷氨酰胺和富含脯氨酸）。
