Narita Miwako, Watanabe Norihiro, Yamahira Akie, Hashimoto Shigeo, Tochiki Nozomi, Saitoh Anri, Kaji Masami, Nakamura Takeshi, Furukawa Tatsuo, Toba Ken, Fuse Ichiro, Aizawa Yoshifusa, Takahashi Masuhiro
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University, Niigata, Japan.
Leuk Res. 2009 Sep;33(9):1224-32. doi: 10.1016/j.leukres.2009.03.047. Epub 2009 May 13.
We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3Ralpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was prominent. Production of IFN-alpha and IL-12 in PMDC05 was enhanced by the stimulation with CpG-A and LPS, respectively. PMDC05 possessed a considerable antigen presenting ability, which was enhanced by culturing with IL3, influenza virus or LPS. PMDC05 could be a useful tool for investigating the pathophysiology of pDCL.
我们从浆细胞样树突状细胞白血病的白血病细胞中建立了一个浆细胞样树突状细胞（pDC）系（PMDC05）。PMDC05细胞在缺乏谱系标志物的情况下，CD4、CD56、CD33、HLA-DR、CD123（IL-3Rα）和CD86呈阳性。TLR1、TLR2、TLR4、TLR7和TLR9的mRNA明显表达，其中TLR7最为突出。分别用CpG-A和LPS刺激可增强PMDC05中IFN-α和IL-12的产生。PMDC05具有相当的抗原呈递能力，通过与IL3、流感病毒或LPS培养可增强该能力。PMDC05可能是研究浆细胞样树突状细胞白血病病理生理学的有用工具。
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