溶剂胁迫对酿酒酵母中MMS诱导的基因变化的影响。

The influence of solvent stress on MMS-induced genetic change in Saccharomyces cerevisiae.

作者信息

Zimmermann F K, Rohlfs A

机构信息

Institut für Mikrobiologie, Technische Hochschule, Darmstadt, F.R.G.

出版信息

Mutat Res. 1991 Sep-Oct;250(1-2):239-49. doi: 10.1016/0027-5107(91)90181-m.

DOI:10.1016/0027-5107(91)90181-m

PMID:1944341

Abstract

MMS induced mitotic recombination but not mitotic chromosome loss when tested in pure form in strain D61.M of Saccharomyces cerevisiae, confirming previous results of Albertini (1991), whereas in Aspergillus nidulans it also induced chromosomal malsegregation in addition to mitotic recombination (Käfer, 1988). However, induction of mitotic chromosome loss was observed in combination with strong inducers of chromosome loss such as the aprotic polar solvents ethyl acetate and to a lesser extent methyl ethyl ketone but not with gamma-valerolactone and propionitrile. In addition to this, 4 solvents, dimethyl formamide, dimethyl sulfoxide, dioxane and pyridine, enhanced the MMS-induced mitotic recombination in strain D61.M. An enhancement of MMS-induced mitotic recombination and reverse mutation could be demonstrated for ethyl acetate and gamma-valerolactone in yeast strain D7.

摘要

当以纯形式在酿酒酵母D61.M菌株中进行测试时，甲磺酸甲酯（MMS）诱导了有丝分裂重组，但未诱导有丝分裂染色体丢失，这证实了阿尔贝蒂尼（1991年）之前的结果；而在构巢曲霉中，除了有丝分裂重组外，它还诱导了染色体错分离（凯费尔，1988年）。然而，在与强染色体丢失诱导剂（如非质子极性溶剂乙酸乙酯以及程度稍轻的甲乙酮）联合使用时，观察到了有丝分裂染色体丢失的诱导现象，但与γ-戊内酯和丙腈联合使用时未观察到。除此之外，4种溶剂，即二甲基甲酰胺、二甲基亚砜、二氧六环和吡啶，增强了D61.M菌株中甲磺酸甲酯诱导的有丝分裂重组。对于乙酸乙酯和γ-戊内酯，在酵母菌株D7中可以证明甲磺酸甲酯诱导的有丝分裂重组和回复突变增强。