细菌细胞中对尺寸扩展的DNA碱基对进行高效复制绕过
Efficient replication bypass of size-expanded DNA base pairs in bacterial cells.
作者信息
Delaney James C, Gao Jianmin, Liu Haibo, Shrivastav Nidhi, Essigmann John M, Kool Eric T
机构信息
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
出版信息
Angew Chem Int Ed Engl. 2009;48(25):4524-7. doi: 10.1002/anie.200805683.
Abstract
Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.
摘要
让我变大！尺寸扩展的DNA碱基（xDNA）能够在复制过程中编码天然DNA序列。用DNA聚合酶进行的体外实验表明，核苷酸能够与xDNA碱基正确配对并掺入。使用大肠杆菌进行的体内实验表明，两个xDNA碱基（xA和xC，见图）编码正确的复制配对碱基。
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本文引用的文献
1
Difluorotoluene, a Nonpolar Isostere for Thymine, Codes Specifically and Efficiently for Adenine in DNA Replication.二氟甲苯，胸腺嘧啶的一种非极性生物电子等排体，在DNA复制中特异性且高效地编码腺嘌呤。
J Am Chem Soc. 1997 Feb 26;119(8):2056-2057. doi: 10.1021/ja963718g.
2
Unnatural substrate repertoire of A, B, and X family DNA polymerases.A、B和X家族DNA聚合酶的非天然底物库
J Am Chem Soc. 2008 Nov 5;130(44):14872-82. doi: 10.1021/ja803833h. Epub 2008 Oct 11.
3
Artificial DNA made exclusively of nonnatural C-nucleosides with four types of nonnatural bases.完全由具有四种非天然碱基的非天然C核苷构成的人工DNA。
J Am Chem Soc. 2008 Jul 9;130(27):8762-8. doi: 10.1021/ja801058h. Epub 2008 Jun 11.
4
In vitro incorporation of LNA nucleotides.锁核酸核苷酸的体外掺入。
Nucleosides Nucleotides Nucleic Acids. 2007;26(8-9):1207-10. doi: 10.1080/15257770701527844.
5
An efficient unnatural base pair for PCR amplification.一种用于PCR扩增的高效非天然碱基对。
J Am Chem Soc. 2007 Dec 19;129(50):15549-55. doi: 10.1021/ja073830m. Epub 2007 Nov 21.
6
An unusual mode of DNA duplex association: Watson-Crick interaction of all-purine deoxyribonucleic acids.一种不同寻常的DNA双链结合模式：全嘌呤脱氧核糖核酸的沃森-克里克相互作用
Chem Biol. 2007 May;14(5):525-31. doi: 10.1016/j.chembiol.2007.03.012.
7
Proficient and accurate bypass of persistent DNA lesions by DinB DNA polymerases.DinB DNA聚合酶对持久性DNA损伤的高效且准确的绕过。
Cell Cycle. 2007 Apr 1;6(7):817-22. doi: 10.4161/cc.6.7.4065. Epub 2007 Apr 23.
8
The model student: what chemical model systems can teach us about biology.模范学生：化学模型系统能让我们了解生物学的哪些方面。
Nat Chem Biol. 2007 Feb;3(2):70-3. doi: 10.1038/nchembio0207-70.
9
Toward a designed, functioning genetic system with expanded-size base pairs: solution structure of the eight-base xDNA double helix.迈向具有扩展碱基对的设计型功能遗传系统：八碱基xDNA双螺旋的溶液结构
J Am Chem Soc. 2006 Nov 15;128(45):14704-11. doi: 10.1021/ja065606n.
10
Unnatural base pair systems for DNA/RNA-based biotechnology.用于基于DNA/RNA的生物技术的非天然碱基对系统。
Curr Opin Chem Biol. 2006 Dec;10(6):622-7. doi: 10.1016/j.cbpa.2006.09.021. Epub 2006 Oct 10.
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