Delaney James C, Gao Jianmin, Liu Haibo, Shrivastav Nidhi, Essigmann John M, Kool Eric T
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Angew Chem Int Ed Engl. 2009;48(25):4524-7. doi: 10.1002/anie.200805683.
Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.
让我变大！尺寸扩展的DNA碱基（xDNA）能够在复制过程中编码天然DNA序列。用DNA聚合酶进行的体外实验表明，核苷酸能够与xDNA碱基正确配对并掺入。使用大肠杆菌进行的体内实验表明，两个xDNA碱基（xA和xC，见图）编码正确的复制配对碱基。
