Zhang Xi-Chun, Kainz Vanessa, Jakubowski Moshe, Burstein Rami, Strassman Andrew, Levy Dan
Department of Anesthesia, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, United States.
Neurosci Lett. 2009 Mar 6;452(1):33-6. doi: 10.1016/j.neulet.2009.01.032.
Primary headaches such as migraine can be aborted by systemic administration of non-steroidal anti-inflammatory drugs (NSAIDs), potentially through the non-selective inhibition of cyclooxygenase (COX) activity in the intracranial meninges. In this study we have used single and double labeling immunohistochemistry to examine the distribution of the COX-1 and COX-2 isoforms in the intracranial dura mater of the rat and identify cell types that express them. COX-1 immunoreactivity was found in medium and small dural blood vessels and was co-expressed with the endothelial cell markers vimentin and the endothelial isoform of nitric oxide synthase (ecNOS). COX-1 was also found to be present in most dural mast cells. COX-2 was mainly expressed in ED2-positive resident dural macrophages. Constitutive COX-2 expression was also found in some axonal profiles, many of which were co-labeled with the nociceptor peptide marker CGRP. The findings suggest that NSAIDs may abort headache, at least in part, by inhibiting either neuronal or non-neuronal COX activity in the dura mater.
诸如偏头痛之类的原发性头痛可通过全身给予非甾体抗炎药（NSAIDs）来缓解，这可能是通过非选择性抑制颅内脑膜中的环氧化酶（COX）活性实现的。在本研究中，我们使用单标和双标免疫组织化学方法来检测COX-1和COX-2同工型在大鼠颅内硬脑膜中的分布，并确定表达它们的细胞类型。在硬脑膜中小血管中发现了COX-1免疫反应性，并且它与内皮细胞标志物波形蛋白和一氧化氮合酶的内皮同工型（ecNOS）共表达。还发现COX-1存在于大多数硬脑膜肥大细胞中。COX-2主要表达于ED2阳性的常驻硬脑膜巨噬细胞中。在一些轴突轮廓中也发现了组成型COX-2表达，其中许多与伤害感受肽标志物降钙素基因相关肽（CGRP）共标记。这些发现表明，NSAIDs可能至少部分地通过抑制硬脑膜中神经元或非神经元的COX活性来缓解头痛。
