呼出气戊烷：肝脏缺血再灌注损伤中脂质过氧化早期及持续监测的指标

Breath pentane: an indicator for early and continuous monitoring of lipid peroxidation in hepatic ischaemia-reperfusion injury.

作者信息

Li Peng, Xu Guowang, Wang Changsong, Gong Yulei, He Ying

机构信息

Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, Harbin, P.R. China.

出版信息

Eur J Anaesthesiol. 2009 Jun;26(6):513-9. doi: 10.1097/eja.0b013e328326f7b7.

DOI:10.1097/eja.0b013e328326f7b7

PMID:19445060

Abstract

BACKGROUND AND OBJECTIVE

Lipid peroxidation plays an important role during liver ischaemia-reperfusion injury. Pentane in breath is often used as an index of lipid peroxidation. We observed the changes in levels of breath pentane during the lipid peroxidation process caused by liver ischaemia-reperfusion injury.

METHODS

Ten male swine were anaesthetized with chloral hydrate 0.3-0.5 g kg(-1) min(-1). Total hepatic ischaemia was induced by occluding the portal inflow vessels. Ischaemia lasted 30 min followed by reperfusion for 180 min. Breath samples were sampled from the anaesthesia circuit and blood samples were collected from the inferior vena cava. Pentane concentrations in breath and blood were quantified by means of solid phase microextraction and gas chromatography-mass spectrography technique.

RESULTS

Exhaled pentane concentrations (means +/- SE) increased markedly after reperfusion for 1 min (244.13 +/- 33.3 pmol l(-1)) and decreased gradually to initial levels after reperfusion for 60 min. Blood pentane concentrations (means +/- SE) increased significantly after reperfusion for 1 min (333.46 +/- 63.05 pmol l(-1)) and then decreased to basal level. Breath pentane concentrations showed a correlation with blood (r = 0.709, P < 0.05).

CONCLUSION

Breath pentane analysis could provide early, rapid, noninvasive and continuous assessment of lipid peroxidation during hepatic ischaemia-reperfusion injury.

摘要

背景与目的

脂质过氧化在肝脏缺血再灌注损伤过程中起重要作用。呼出气体中的戊烷常被用作脂质过氧化的指标。我们观察了肝脏缺血再灌注损伤所致脂质过氧化过程中呼出戊烷水平的变化。

方法

选用10只雄性猪，用0.3 - 0.5 g·kg⁻¹·min⁻¹的水合氯醛麻醉。通过阻断门静脉入流诱导全肝缺血。缺血持续30分钟，随后再灌注180分钟。从麻醉回路采集呼气样本，从下腔静脉采集血样。采用固相微萃取和气相色谱 - 质谱联用技术定量测定呼气和血液中的戊烷浓度。

结果

再灌注1分钟后呼出戊烷浓度（均值±标准误）显著升高（244.13±33.3 pmol·l⁻¹），再灌注60分钟后逐渐降至初始水平。再灌注1分钟后血中戊烷浓度（均值±标准误）显著升高（333.46±63.05 pmol·l⁻¹），然后降至基础水平。呼出戊烷浓度与血液中戊烷浓度呈相关性（r = 0.709，P < 0.05）。