用于粘质沙雷氏菌分离株指纹图谱分析的快速脉冲场凝胶电泳方法。
Rapid PFGE method for fingerprinting of Serratia marcescens isolates.
作者信息
Alaidan Alwaleed, Bohol Marie Fe, Al-Thawadi Sahar I, Al-Suwaine Abdulrahman, Al-Qahtani Ahmed A, Al-Ahdal Mohammed N
机构信息
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
出版信息
J Microbiol Methods. 2009 Aug;78(2):238-41. doi: 10.1016/j.mimet.2009.05.007. Epub 2009 May 13.
PMID:19446583
Abstract
We modified the conventional PFGE procedure for Serratia marcescens to establish a rapid method. Our protocol showed modification in the bacterial lysis, washing, and restriction enzyme digestion time. This resulted in shortening the time needed by about 3 days compared to the conventional PFGE method.
摘要
我们对粘质沙雷氏菌的传统脉冲场凝胶电泳（PFGE）程序进行了改进，以建立一种快速方法。我们的方案在细菌裂解、洗涤和限制性内切酶消化时间方面进行了改进。与传统的PFGE方法相比，这使得所需时间缩短了约3天。
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