Nakayama T, Sugae K, Narita K
J Biochem. 1977 Jun;81(6):1803-12. doi: 10.1093/oxfordjournals.jbchem.a131641.
Washed, preincubated minced hen's oviducts, which contained low levels of extracellular and intracellular proteins, synthesized egg-white proteins actively. The addition of ovalbumin to the incubation medium resulted in inhibition of the synthesis of egg-white proteins by the washed, preincubated oviduct cells, while the addition of bovine serum albumin seemed to stimulate protein synthesis and hen's egg-white lysozyme had no effect. The inhibitory or stimulatory effect on protein synthesis was proportional to the amount of protein added to the medium. The inhibitory effect of added ovalbumin was shown not to be due to the incorporation of ovalbumin into the oviduct cells from the incubation medium. Egg-white proteins added to the medium also inhibited protein synthesis inside the cells and the extent of the inhibition appeared to correspond to the amount of ovalbumin present in egg-white.
经过洗涤和预孵育的切碎母鸡输卵管，其细胞外和细胞内蛋白质含量较低，但仍能积极合成蛋清蛋白。向孵育培养基中添加卵清蛋白会导致经过洗涤和预孵育的输卵管细胞对蛋清蛋白合成产生抑制作用，而添加牛血清白蛋白似乎会刺激蛋白质合成，且鸡蛋清溶菌酶则没有影响。对蛋白质合成的抑制或刺激作用与添加到培养基中的蛋白质量成正比。已表明添加的卵清蛋白的抑制作用并非源于卵清蛋白从孵育培养基中掺入输卵管细胞。添加到培养基中的蛋清蛋白也会抑制细胞内的蛋白质合成，且抑制程度似乎与蛋清中存在的卵清蛋白量相对应。
