Nakayama T, Narita K
J Biochem. 1977 Jan;81(1):153-62. doi: 10.1093/oxfordjournals.jbchem.a131430.
The minced oviducts prepared from laying hens contained considerable amounts of extracellular proteins (about 60 mg per g of the wet oviducts), which could largely be removed (about 80%) by washing four times with chilled 5rebs-Ringer-Tris buffer, pH 7.6. When the washed, minced oviducts were incubated at 37 degrees with the buffer, intracellular proteins were increasingly secreted to the extracellular fluids as the incubation time was increased and the amounts of intracellular proteins decreased concomitatnly, suggesting that the rate of secretion of intracellular proteins was faster than that of protein synthesis under the conditions used (without addition of amino acids to the medium). Repeated 5-min incubations (five to seven times) resulted in the secretion of intracellular proteins in amounts of 100 mg or more per g of the wet oviducts. The secretion rate of intracellular proteins was almost constant at 77 degrees regardless of the volume of buffer used for the incubation, and no secretion occurred at 4 degrees even after 5 h. It appeared that a 30 min period was required for the secretion of intracellular proteins from the oviducts at 37 degrees and that the rate of secretion was not affected appreciably by the amount of secreted proteins present in the extracellular fluids. The protein synthesis activity in the washed, preincubated minced oviducts was independent of the amount of intracellular proteins involved but was dependent upon that of secreted proteins present in the extracellular fluids.
由产蛋母鸡制备的切碎的输卵管含有大量细胞外蛋白质（每克湿输卵管约60毫克），通过用pH 7.6的冰冷5rebs-林格- Tris缓冲液洗涤四次，这些蛋白质大部分（约80%）可以被去除。当洗涤后的切碎输卵管与缓冲液在37℃孵育时，随着孵育时间增加，细胞内蛋白质越来越多地分泌到细胞外液中，同时细胞内蛋白质的量相应减少，这表明在所使用的条件下（培养基中不添加氨基酸），细胞内蛋白质的分泌速率比蛋白质合成速率快。重复进行5分钟的孵育（五到七次）导致每克湿输卵管分泌100毫克或更多的细胞内蛋白质。细胞内蛋白质的分泌速率在77℃时几乎恒定，与用于孵育的缓冲液体积无关，在4℃时即使孵育5小时也不发生分泌。在37℃下，输卵管分泌细胞内蛋白质似乎需要30分钟，并且分泌速率不受细胞外液中存在的分泌蛋白质数量的明显影响。洗涤过的、预孵育的切碎输卵管中的蛋白质合成活性与所涉及的细胞内蛋白质数量无关，但取决于细胞外液中存在的分泌蛋白质数量。
