Di Gioia Diana, Sciubba Luigi, Bertin Lorenzo, Barberio Claudia, Salvadori Laura, Frassinetti Stefania, Fava Fabio
Department of Applied Chemistry and Material Science, University of Bologna, Via Terracini 28, 40131 Bologna, Italy.
Water Res. 2009 Jul;43(12):2977-88. doi: 10.1016/j.watres.2009.04.011. Epub 2009 Apr 19.
An aerobic bacterial consortium (Consortium A) was recently obtained from textile wastewater and was capable of degrading 4-nonylphenol and nonylphenol polyethoxylates (NPnEOs). In the perspective of developing a biotechnological process for the treatment of effluents from activated sludge plants fed with NPnEO contaminated wastewater, the capability of Consortium A of biodegrading an industrial mixture of NPnEOs in the physiological condition of immobilized cells was investigated. Two identically configured packed bed reactors were developed by immobilizing the consortium on silica beads or granular activated carbon. Both reactors were tested in batch and continuous mode by feeding them with water supplemented with NPnEOs. The two reactors were monitored through chemical, microbiological and molecular integrated methodology. Active biofilms were generated on both immobilization supports. Both reactors displayed comparable NPnEO mineralization under batch and continuous conditions. FISH analyses evidenced that the biofilms evolved with time by changing the reactor operation mode and the organic load. Taken together, the data collected in this study provide a preliminary strong indication on the feasibility of Consortium A-based biofilm technology for the decontamination of NPnEO containing effluents.
最近从纺织废水中获得了一个好氧细菌群落（群落A），它能够降解4-壬基酚和壬基酚聚乙氧基化物（NPnEOs）。从开发一种生物技术工艺用于处理以NPnEO污染废水为进水的活性污泥厂的出水的角度出发，研究了群落A在固定化细胞生理条件下对NPnEOs工业混合物的生物降解能力。通过将该群落固定在硅胶珠或颗粒活性炭上，开发了两个配置相同的填充床反应器。通过向两个反应器中加入补充了NPnEOs的水，以分批和连续模式对它们进行测试。通过化学、微生物和分子综合方法对这两个反应器进行监测。在两种固定化载体上均形成了活性生物膜。在分批和连续条件下，两个反应器均表现出相当的NPnEO矿化能力。荧光原位杂交（FISH）分析表明，生物膜会随着时间的推移，通过改变反应器运行模式和有机负荷而发生演变。综上所述，本研究收集的数据为基于群落A的生物膜技术用于含NPnEO废水去污的可行性提供了初步的有力证据。
