Rodríguez C, Medici M, Rodríguez A V, Mozzi F, Font de Valdez G
Centro de Referencia para Lactobacilos-CONICET, Chacabuco 145, San Miguel de Tucumán, 4000 Tucumán, Argentina.
J Dairy Sci. 2009 Jun;92(6):2423-34. doi: 10.3168/jds.2008-1724.
Acetyl-salicylic acid (ASA) is a nonsteroidal antiinflammatory/analgesic drug, which may cause gastritis or stomach ulcers if intensively employed. Exopolysaccharide (EPS)-producing lactic acid bacteria have been claimed to induce immunostimulatory/antiulcer effects in the host. This study investigated the potential preventive effect of fermented milks (FM) with EPS-producing Streptococcus thermophilus strains (CRL 1190 and CRL 804) on an in vivo model of chronic gastritis. Fermented milks (2 EPS(+) and 1 EPS(-), separately) were fed to BALB/c mice for 7 d before inducing gastritis with ASA (400 mg/kg of body weight per day for 10 d; gastritis group, n = 5). Appropriate control groups (ASA administered but not given FM, n = 5; and ASA not administered but given FM) were included in this study. Gastric inflammatory activity was evaluated through the stomach's histology and the number of IFNgamma(+) and IL-10(+) cytokine-producing cells in the gastric mucosa. Only mice preventively treated with the EPS-producing Strep. thermophilus CRL 1190 FM and later administered ASA did not develop gastritis, showing a conserved gastric mucosa structure similar to those of healthy mice. A marked decrease of IFNgamma(+)- and increase of IL-10(+)-producing cells compared with the gastritis group mice were observed. Purified EPS from Strep. thermophilus CRL 1190 resuspended in autoclaved milk was also effective for gastritis prevention. The EPS-protein interaction might be responsible for the observed gastroprotective effect; such interactions may be affected by industrial manufacturing conditions. The results indicate that the FM with Strep. thermophilus CRL 1190 or its EPS could be used in novel functional foods for preventing chronic gastritis.
乙酰水杨酸（ASA）是一种非甾体抗炎/镇痛药，如果大量使用可能会导致胃炎或胃溃疡。据称，产生胞外多糖（EPS）的乳酸菌可在宿主体内诱导免疫刺激/抗溃疡作用。本研究调查了含有产EPS嗜热链球菌菌株（CRL 1190和CRL 804）的发酵乳（FM）对慢性胃炎体内模型的潜在预防作用。在用ASA诱导胃炎之前（每天400mg/kg体重，持续10天；胃炎组，n = 5），将发酵乳（分别为2种EPS（+）和1种EPS（-））喂给BALB/c小鼠7天。本研究纳入了适当的对照组（给予ASA但未给予FM，n = 5；未给予ASA但给予FM）。通过胃组织学以及胃黏膜中产生IFNγ（+）和IL-10（+）细胞因子的细胞数量来评估胃炎症活性。只有预先用产EPS的嗜热链球菌CRL 1190发酵乳处理并随后给予ASA的小鼠未发生胃炎，其胃黏膜结构与健康小鼠相似。与胃炎组小鼠相比，观察到产生IFNγ（+）的细胞明显减少，产生IL-10（+）的细胞增加。将嗜热链球菌CRL 1190纯化的EPS重悬于高压灭菌的牛奶中对胃炎预防也有效。EPS - 蛋白质相互作用可能是观察到的胃保护作用的原因；这种相互作用可能会受到工业生产条件的影响。结果表明，含有嗜热链球菌CRL 1190或其EPS的发酵乳可用于新型功能性食品中以预防慢性胃炎。
