Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, San Miguel de Tucumán, 4000, Tucumán, Argentina.
World J Gastroenterol. 2010 Apr 7;16(13):1622-30. doi: 10.3748/wjg.v16.i13.1622.
To investigate the potential therapeutic effect of exopolysaccharide (EPS)-producing Streptococcus thermophilus (S. thermophilus) CRL 1190 fermented milk on chronic gastritis in Balb/c mice.
Balb/c mice were fed with the fermented milk for 7 d after inducing gastritis with acetyl-salicylic acid (ASA, 400 mg/kg body weight per day for 10 d). Omeprazole was included in this study as a positive therapeutic control. The gastric inflammatory activity was evaluated from gastric histology and inflammation score, number of interleukin-10 (IL-10), interferon-gamma (INFgamma) and tumor necrosis factor-alpha (TNF-alpha) cytokine-producing cells in the gastric mucosa, and thickness of the mucus layer.
Animals receiving treatment with the EPS-producing S. thermophilus CRL 1190 fermented milk showed a conserved gastric mucosa structure similar to that of healthy animals. Inflammation scores of the fermented milk-treated mice were lower than those of mice in the gastritis group (0.2 + or - 0.03 vs 2.0 + or - 0.6, P < 0.05). A marked decrease in INFgamma(+) (15 + or - 1.0 vs 28 + or - 1.2, P < 0.05) and TNF-alpha(+) (16 + or - 3.0 vs 33 + or - 3.0, P < 0.05) cells and an increase in IL-10(+) (28 + or - 1.5 vs 14 + or - 1.3, P < 0.05) cells compared to the gastritis group, was observed. Also, an increase in the thickness of the mucus gel layer (2.2 + or - 0.6 vs 1.0 + or - 0.3; 5.1 + or - 0.8 vs 1.5 + or - 0.4 in the corpus and antrum mucosa, respectively, P < 0.05) compared with the gastritis group was noted. A milk suspension of the purified EPS from S. thermophilus CRL1190 was also effective as therapy for gastritis.
This study suggests that fermented milk with S. thermophilus CRL 1190 and/or its EPS could be used in novel functional foods as an alternative natural therapy for chronic gastritis induced by ASA.
研究产胞外多糖（EPS）的嗜热链球菌（S. thermophilus）CRL 1190发酵乳对 Balb/c 小鼠慢性胃炎的潜在治疗作用。
用乙酰水杨酸（ASA，每天 400mg/kg 体重，共 10 天）诱导胃炎后，Balb/c 小鼠用发酵乳喂养 7 天。本研究中包括奥美拉唑作为阳性治疗对照。从胃组织学和炎症评分、胃黏膜中白细胞介素 10（IL-10）、干扰素-γ（INFgamma）和肿瘤坏死因子-α（TNF-alpha）细胞的数量以及黏液层厚度评估胃的炎症活性。
接受产 EPS 的 S. thermophilus CRL 1190 发酵乳治疗的动物胃黏膜结构与健康动物相似。与胃炎组小鼠相比，发酵乳治疗组小鼠的炎症评分较低（0.2 ± 0.03 对 2.0 ± 0.6，P < 0.05）。INFgamma（+）（15 ± 1.0 对 28 ± 1.2，P < 0.05）和 TNF-alpha（+）（16 ± 3.0 对 33 ± 3.0，P < 0.05）细胞数量明显减少，IL-10（+）细胞数量增加（28 ± 1.5 对 14 ± 1.3，P < 0.05）。与胃炎组相比，胃黏膜黏液凝胶层厚度增加（胃体和胃窦黏膜分别为 2.2 ± 0.6 对 1.0 ± 0.3；5.1 ± 0.8 对 1.5 ± 0.4，P < 0.05）。从 S. thermophilus CRL1190 中分离出的纯化 EPS 牛奶悬液也可有效治疗胃炎。
本研究表明，含嗜热链球菌 CRL 1190 和/或其 EPS 的发酵乳可作为新型功能性食品用于治疗 ASA 诱导的慢性胃炎，是一种天然替代疗法。
