Renò Filippo, Traina Vincenzina, Cannas Mario
Research Centre for Biocompatibility and Tissue Engineering, Clinical and Experimental Medicine Department, University of Eastern Piedmont A. Avogadro, Novara, Italy.
Cell Adh Migr. 2009 Jul-Sep;3(3):239-42. doi: 10.4161/cam.3.3.8632. Epub 2009 Jul 1.
Cells within human skin are exposed to mechanical stretching that is considered a trigger stimulus for keratinocyte proliferation, while its effect on keratinocyte migration has been poorly investigate. In order to explore the effect of stretching on keratinocyte migration spontaneously immortalized human keratinocyte (HaCaT) monolayers seeded onto collagen I-coated silicon sheets were stimulated 3 times for 1 hour every 24 hours (total time = 72 hours) by mechanical stretching increasing substrate deformations (10%) applied both as static (0 Hz) and cyclic (0.17 Hz) uniaxial stretching. At the end of stimulations monolayer areas measured in both static and cyclic samples appeared reduced and strongly oriented in a direction perpendicular to the stress direction compared to unstimulated ones. Moreover during the mechanical stimulation period HaCaT monolayers strongly increased the release in the medium of matrix metalloproteinase 9 (MMP-9), a proteolytic enzyme necessary for keratinocyte migration.
人类皮肤中的细胞会受到机械拉伸，这种拉伸被认为是角质形成细胞增殖的触发刺激因素，而其对角质形成细胞迁移的影响尚未得到充分研究。为了探究拉伸对角质形成细胞迁移的影响，将接种在I型胶原蛋白包被的硅片上的自发永生化人类角质形成细胞（HaCaT）单层，每24小时进行3次、每次1小时的机械拉伸刺激（总时长 = 72小时），通过增加底物变形（10%）来施加静态（0 Hz）和循环（0.17 Hz）单轴拉伸。在刺激结束时，与未刺激的样本相比，在静态和循环样本中测量的单层面积均出现减小，并且在垂直于应力方向上强烈定向。此外，在机械刺激期间，HaCaT单层显著增加了基质金属蛋白酶9（MMP-9，角质形成细胞迁移所必需的一种蛋白水解酶）在培养基中的释放。
