毛果芸香碱诱导的癫痫大鼠海马中APLP1 mRNA表达下调。
Down-regulation of APLP1 mRNA expression in hippocampus of pilocarpine-induced epileptic rats.
作者信息
Wang Cheng, You Zi-Li, Zhang Ding-Ding
机构信息
Key Laboratory for Neuroinformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
出版信息
Neurosci Bull. 2009 Jun;25(3):109-14. doi: 10.1007/s12264-009-1229-0.
OBJECTIVE
To investigate the expression of amyloid beta precursor-like protein 1(APLP1) gene on the transcription level in hippocampus of pilocarpine-induced epileptic rats.
METHODS
Epileptic rats were developed by LiC1 (3 mmol/kg, i.p.) approximately 20 h prior to pilocarpine (30 mg/kg, i.p.) administration. The 3' end partial sequence of rat APLP1 gene was cloned, and the expression levels of APLP1 mRNA in hippocampus of epileptic rats at 6 h, 30 h, 7 d and 15 d were determined by semi-quantitative RT-PCR.
RESULTS
The 3'end partial sequence of rat APLP1 gene shared a 97% homology with that of mice, and 90% with that of human. The APLP1 amino acid sequence of rat was identical with that of mouse, but was different from that of human in 3 residues. Moreover, pilocarpine induced a significant down-regulation of APLP1 mRNA expression at 6 h after epilepsy initiation (P< 0.05), and at 30 h, this down-regulation became more dramatic (P< 0.01), which lasted till day 15 (P< 0.01).
CONCLUSION
The 3' end of APLP1 gene is highly conserved, and APLP1 mRNA expression is kept at low level in hippocampus of pilocarpine-induced epileptic rats.
目的
研究毛果芸香碱致痫大鼠海马中淀粉样前体蛋白样蛋白1（APLP1）基因在转录水平的表达。
方法
在腹腔注射毛果芸香碱（30 mg/kg）前约20小时腹腔注射LiC1（3 mmol/kg）制备癫痫大鼠。克隆大鼠APLP1基因3'端部分序列，采用半定量逆转录聚合酶链反应（RT-PCR）检测癫痫大鼠海马在6小时、30小时、7天和15天时APLP1 mRNA的表达水平。
结果
大鼠APLP1基因3'端部分序列与小鼠的同源性为97%，与人类的同源性为90%。大鼠APLP1氨基酸序列与小鼠相同，但与人类在3个残基处不同。此外，癫痫发作后6小时毛果芸香碱诱导APLP1 mRNA表达显著下调（P<0.05），30小时时这种下调更为明显（P<0.01），并持续至第15天（P<0.01）。
结论
APLP1基因3'端高度保守，在毛果芸香碱致痫大鼠海马中APLP1 mRNA表达维持在低水平。
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