细胞培养基中尿素浓度的定量测定。
Quantitative determination of urea concentrations in cell culture medium.
作者信息
Zawada Robert J X, Kwan Peggy, Olszewski Kellen L, Llinas Manuel, Huang Shu-Gui
机构信息
BioAssay Systems LLC, Hayward, CA, USA.
出版信息
Biochem Cell Biol. 2009 Jun;87(3):541-4. doi: 10.1139/o09-011.
Abstract
Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.
摘要
尿素是哺乳动物蛋白质代谢的主要含氮终产物。在此，我们描述了一种使用改良的荣格试剂测定尿素的定量、灵敏方法。该测定法对尿素具有特异性，不受氨（组织和细胞培养中常见的干扰物）的影响。我们证明，这种基于比色微孔板的便捷室温测定法可用于测定细胞培养中的尿素合成。
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