子午面实时裂隙扫描显微镜检查
Real-time slit scanning microscopy in the meridional plane.
作者信息
Botcherby Edward J, Booth Martin J, Juskaitis Rimas, Wilson Tony
机构信息
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.
出版信息
Opt Lett. 2009 May 15;34(10):1504-6. doi: 10.1364/ol.34.001504.
The standard microscope architecture around which confocal microscopes are built imposes fundamental restrictions on the speed with which images (three-dimensional data sets) can be obtained. Commercially available slit scanning confocal microscopes are able to produce optically sectioned images at frame rates well in excess of 100 Hz. However only the focal (x-y) plane can be imaged at this speed. To image a volume specimen it is necessary to physically change the distance between the objective lens and the specimen. This refocusing process is often necessarily slow and represents a bottleneck to the speed of image acquisition. We describe the construction of a slit scanning confocal microscope based on what we know to be a novel microscope architecture, which permits images of other planes and, particular, the meridional (x-z) plane to be acquired in real time.
共聚焦显微镜所基于的标准显微镜架构对获取图像(三维数据集)的速度施加了基本限制。市售的狭缝扫描共聚焦显微镜能够以远超过100Hz的帧率生成光学切片图像。然而,只有焦平面(x-y平面)能够以这种速度成像。要对体积样本进行成像,就必须物理改变物镜与样本之间的距离。这种重新聚焦过程通常必然很慢,并且是图像采集速度的一个瓶颈。我们描述了一种基于我们所知的新型显微镜架构构建的狭缝扫描共聚焦显微镜,它允许实时获取其他平面尤其是子午面(x-z平面)的图像。