Leite João Jaime Giffoni, Brito Erika Helena Salles, Cordeiro Rossana Aguiar, Brilhante Raimunda Sâmia Nogueira, Sidrim José Júlio Costa, Bertini Luciana Medeiros, Morais Selene Maia de, Rocha Marcos Fábio Gadelha
Centro Especializado em Micologia Médica, Universidade Federal do Ceará, Fortaleza, CE.
Rev Soc Bras Med Trop. 2009 Mar-Apr;42(2):110-3. doi: 10.1590/s0037-86822009000200003.
The present study had the aim of testing the hexane and methanol extracts of avocado seeds, in order to determine their toxicity towards Artemia salina, evaluate their larvicidal activity towards Aedes aegypti and investigate their in vitro antifungal potential against strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis through the microdilution technique. In toxicity tests on Artemia salina, the hexane and methanol extracts from avocado seeds showed LC50 values of 2.37 and 24.13 mg mL-1 respectively. Against Aedes aegypti larvae, the LC50 results obtained were 16.7 mg mL-1 for hexane extract and 8.87 mg mL-1 for methanol extract from avocado seeds. The extracts tested were also active against all the yeast strains tested in vitro, with differing results such that the minimum inhibitory concentration of the hexane extract ranged from 0.625 to 1.25mg L-(1), from 0.312 to 0.625 mg mL-1 and from 0.031 to 0.625 mg mL-1, for the strains of Candida spp, Cryptococcus neoformans and Malassezia pachydermatis, respectively. The minimal inhibitory concentration for the methanol extract ranged from 0.125 to 0.625 mg mL-1, from 0.08 to 0.156 mg mL-1 and from 0.312 to 0.625 mg mL-1, for the strains of Candida spp., Cryptococcus neoformans and Malassezia pachydermatis, respectively.
本研究旨在测试鳄梨种子的己烷提取物和甲醇提取物，以确定它们对卤虫的毒性，评估它们对埃及伊蚊的杀幼虫活性，并通过微量稀释技术研究它们对白色念珠菌、新型隐球菌和厚皮马拉色菌菌株的体外抗真菌潜力。在卤虫毒性试验中，鳄梨种子的己烷提取物和甲醇提取物的LC50值分别为2.37和24.13 mg mL-1。对于埃及伊蚊幼虫，鳄梨种子己烷提取物的LC50结果为16.7 mg mL-1，甲醇提取物的LC50结果为8.87 mg mL-1。所测试的提取物在体外对所有测试的酵母菌株也有活性，结果不同，使得己烷提取物对白色念珠菌、新型隐球菌和厚皮马拉色菌菌株的最低抑菌浓度分别为0.625至1.25mg L-(1)、0.312至0.625 mg mL-1和0.031至0.625 mg mL-1。甲醇提取物对白色念珠菌、新型隐球菌和厚皮马拉色菌菌株的最低抑菌浓度分别为0.125至0.625 mg mL-1、0.08至0.156 mg mL-1和0.312至0.625 mg mL-1。
