Department of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799, USA.
J Mater Sci Mater Med. 2009 Oct;20(10):2155-66. doi: 10.1007/s10856-009-3769-2. Epub 2009 May 18.
We investigated the inflammatory response in pigs exposed to salmon fibrinogen/thrombin dressings. Animals were exposed to the material in 3 ways: (a) thrombin and fibrinogen were injected intravenously, (b) dual full-thickness skin lesions were surgically created on the dorsal aspect of the swine and treated with the fibrinogen/thrombin bandage and a commercial bandage or (c) a fibrinogen/thrombin bandage was inserted through an abdominal incision into the peritoneal cavity. Blood was collected twice weekly and animals were sacrificed at 7, 10 or 28 days. Animals in the 28-day dermal lesion group were given an injection of salmon fibrinogen/thrombin at the 10 day point to simulate a second bandage application. The immune response manifested itself as induction of germinal centers in mesenteric lymph nodes and in the white pulp of the spleen. Examination of the histology of the skin and organs showed a cellular inflammatory response with granulation tissue and signs of edema that resolved by the 28-day stage. Antibodies reactive to salmon and human thrombin and fibrinogen were detected, but fibrinogen levels and coagulation processes were not affected. In conclusion, animals treated with salmon fibrinogen/thrombin bandages demonstrated a smooth recovery course in terms of both tissue healing and the immune response without adverse effects from the exposure to the fish proteins.
我们研究了暴露于鲑鱼纤维蛋白原/凝血酶敷贴剂的猪的炎症反应。动物通过 3 种方式暴露于该材料：（a）静脉内注射凝血酶和纤维蛋白原，（b）通过手术在猪的背部创建双重全层皮肤损伤，并使用纤维蛋白原/凝血酶绷带和商业绷带进行治疗，或（c）将纤维蛋白原/凝血酶绷带通过腹部切口插入到腹腔中。每周采集两次血液，并在第 7、10 或 28 天处死动物。在 28 天的皮肤损伤组中的动物在第 10 天点接受鲑鱼纤维蛋白原/凝血酶注射以模拟第二次绷带应用。免疫反应表现为肠系膜淋巴结和脾脏白髓中生发中心的诱导。对皮肤和器官的组织学检查显示出伴有肉芽组织和水肿迹象的细胞炎症反应，到第 28 天阶段得到缓解。检测到针对鲑鱼和人凝血酶和纤维蛋白原的抗体，但纤维蛋白原水平和凝血过程不受影响。总之，用鲑鱼纤维蛋白原/凝血酶绷带治疗的动物在组织愈合和免疫反应方面均表现出平稳的恢复过程，没有因暴露于鱼蛋白而产生不良反应。
