Rothwell Stephen W, Settle Timothy, Wallace Shannon, Dorsey Jennifer, Simpson David, Bowman James R, Janmey Paul, Sawyer Evelyn
Department of Anatomy, Physiology and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Biologicals. 2010 Nov;38(6):619-28. doi: 10.1016/j.biologicals.2010.07.001. Epub 2010 Aug 11.
Experimental salmon thrombin/fibrinogen dressings have been shown to provide effective hemostasis in severe hemorrhage situations. The hypothesis for this study was that swine would still remain healthy without coagulopathy six months after exposure to salmon thrombin/fibrinogen dressings. Initial exposure was by insertion of the salmon dressing into the peritoneal cavity. Three months after the initial exposure, the same animals were subjected to two full thickness dermal wounds on the dorsal surface. One wound was bandaged with the salmon thrombin/fibrinogen bandage and the other wound was dressed with a standard bandage. The animals were monitored for an additional three months. Blood was drawn every 14 days over the six months for immunological and coagulation function analysis. All of the animals (8 pigs) remained healthy during the six month period and the dermal wounds healed without incidence. Lymph nodes and spleen showed signs of normal immune response and Western blots showed development of antibodies against salmon fibrinogen, but none of the animals made antibodies that recognized any species of thrombin. Coagulation parameters (fibrinogen concentration, thrombin time, PT and aPTT) and hematological parameters remained normal over the course of the study when compared to initial values of the subject swine.
实验性鲑鱼凝血酶/纤维蛋白原敷料已被证明在严重出血情况下能有效止血。本研究的假设是，猪在接触鲑鱼凝血酶/纤维蛋白原敷料六个月后仍能保持健康且无凝血病。初次接触是通过将鲑鱼敷料插入腹腔。初次接触三个月后，对同一批动物的背部进行两处全层皮肤创伤。一处伤口用鲑鱼凝血酶/纤维蛋白原绷带包扎，另一处伤口用标准绷带包扎。对动物再监测三个月。在六个月期间，每14天采集一次血液进行免疫和凝血功能分析。所有动物（8头猪）在六个月期间均保持健康，皮肤伤口愈合且无并发症。淋巴结和脾脏显示出正常免疫反应的迹象，蛋白质印迹显示产生了针对鲑鱼纤维蛋白原的抗体，但没有动物产生能识别任何种类凝血酶的抗体。与受试猪的初始值相比，在研究过程中凝血参数（纤维蛋白原浓度、凝血酶时间、PT和活化部分凝血活酶时间）和血液学参数均保持正常。
