高效液相色谱-光电二极管阵列检测法测定白薇中4种苯乙酮类成分

[Determination of four acetophenones in Radix Cynanchi bungei by high performance liquid chromatography-photodiode array detection].

作者信息

Sun Yinshi, Liu Zhengbo, Wang Jianhua, Wang Ying, Zhou Hongying, Zhang Wenli, Wang Jun, Qiao Gui

机构信息

College of Agronomy, Shandong Agricultural University, Taian 271018, China.

出版信息

Se Pu. 2009 Jan;27(1):114-6.

PMID:19449554

Abstract

A high performance liquid chromatographic (HPLC) method was developed for the determination of four acetophenones, namely 4-hydroxyacetophenone (I), 2,5-dihydroxyacetophenone (II), baishouwubenzophenone (III) and 2,4-dihydroxyacetophenone (IV), in Radix Cynanchi bungei. The HPLC with photodiode array (PDA) detection was carried out on a Symmetry-C18 (4.6 mm x 250 mm, 5 microm) column with CH3OH-H2O (26 : 74, v/v) as the mobile phase at the flow rate of 1.0 mL/min and 30 degrees C. The compounds I , III, IV were monitored at 280 nm, and the compound II was at 224 nm. Four acetophenones (I - IV) had good linearities (r = 0.999 6 - 0.999 9) at the ranges of 0.080 - 0.560 microg, 0.080 -0.560 microg, 0.100 - 0.700 microg and 0.092 -0.644 microg, respectively. The average recoveries were from 98.0% to 104.0% with relative standard deviations (RSD) ranging from 0.8% to 2.6%. The method is of quick, simple and accurate. The method can be used for the quality control of this product.

摘要

建立了一种高效液相色谱（HPLC）法，用于测定白首乌中4-羟基苯乙酮（I）、2,5-二羟基苯乙酮（II）、白首乌二苯酮（III）和2,4-二羟基苯乙酮（IV）这4种苯乙酮类成分。采用配有光电二极管阵列（PDA）检测器的HPLC，在Symmetry-C18（4.6 mm×250 mm，5μm）色谱柱上进行分析，以甲醇-水（26∶74，v/v）为流动相，流速为1.0 mL/min，柱温为30℃。化合物I、III、IV在280 nm波长处进行监测，化合物II在224 nm波长处进行监测。4种苯乙酮类成分（I - IV）在0.080 - 0.560μg、0.080 - 0.560μg、0.100 - 0.700μg和0.092 - 0.644μg范围内均具有良好的线性关系（r = 0.999 6 - 0.999 9）。平均回收率为98.0%至104.0%，相对标准偏差（RSD）为0.8%至2.6%。该方法快速、简便、准确，可用于该产品的质量控制。