Ni Wan-min, Zhang Hang-jun, Zhang Jian-ying, Yao Chao-ying
Environment and Ecology Research Center, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China.
Ying Yong Sheng Tai Xue Bao. 2009 Jan;20(1):228-32.
Aiming at the combined pollution of low dose microcystins (MCs) and disinfection byproducts (DBPs) in drinking water, the individual and combined cytotoxic effects of two MCs (MCLR and MCRR) and two DBP compounds (CHClBr1 and CHCl2Br) on the apoptosis of isolated grass carp (Ctenopharngodon idellus) lymphocytes were examined by using in vitro bioassays. The results showed that after 2 hours exposure to the four pollutants within the range of test concentrations, the apoptosis of isolated lymphocyte occurred, and there existed significant dose-effect relationship. The combined cytotoxic effects of 1 nmol x L(-1) MCLR or MCRR and 1-100 nmol x L(-1) CHClr2 or CHCl2Br were all of additive, and the apoptosis percent was highly correlated with the exposure dose. It was suggested that the apoptosis percent of grass carp lymphocytes could be used as an effective indicator to evaluate the cytotoxicity caused by the combined pollution of MCs and DBPs.
针对饮用水中低剂量微囊藻毒素（MCs）与消毒副产物（DBPs）的复合污染问题，采用体外生物测定法，研究了两种微囊藻毒素（MCLR和MCRR）和两种消毒副产物化合物（CHClBr1和CHCl2Br）对离体草鱼（Ctenopharngodon idellus）淋巴细胞凋亡的单独及联合细胞毒性效应。结果表明，在试验浓度范围内，经4种污染物暴露2小时后，离体淋巴细胞发生凋亡，且存在显著的剂量效应关系。1 nmol·L⁻¹ MCLR或MCRR与1 - 100 nmol·L⁻¹ CHClr2或CHCl2Br的联合细胞毒性效应均为相加作用，凋亡率与暴露剂量高度相关。提示草鱼淋巴细胞凋亡率可作为评价MCs与DBPs复合污染所致细胞毒性的有效指标。
