Fang Wen-Di, Zhang Hang-Jun, Wu Yu-Huan
Ying Yong Sheng Tai Xue Bao. 2014 Feb;25(2):584-90.
In this study, grass carp (Ctenopharyngodon idellus) lymphocytes were used as the vitro test object to demonstrate the joint effects of microcystins (MC-LR) and bacterial lipopolysaccharides (LPS) on fish immune system. The results showed that MC-LR and LPS in the single and combined exposure groups could both induce grass carp lymphocytes apoptosis with typical ladder-like DNA electrophoresis characteristics. However, comparing the apoptosis rate of the combined and single exposure groups, it was suggested that bacterial LPS could cooperate with MC-LR causing a higher rate of fish lymphocytes apoptosis (2.1 and 3.3-fold of that for the single exposure group I (MC-LR) and II (LPS), respectively), and there existed a significant dose-response relationship. The MC-LR cooperating with bacterial LPS decreased the activity of glutathione S-transferase (GST), increased the levels of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA), resulted in DNA damage and cell arrest in G0 phase, which inhibited cell proliferation and accelerated apoptosis. It was proved that MC-LR exacerbated fish immunotoxicity by collaborating with LPS, which had a serious adverse effect on aquaculture industry.
在本研究中，以草鱼（Ctenopharyngodon idellus）淋巴细胞作为体外试验对象，以证明微囊藻毒素（MC-LR）和细菌脂多糖（LPS）对鱼类免疫系统的联合作用。结果表明，单暴露组和联合暴露组中的MC-LR和LPS均可诱导草鱼淋巴细胞凋亡，具有典型的梯状DNA电泳特征。然而，比较联合暴露组和单暴露组的凋亡率，结果表明细菌LPS可与MC-LR协同作用，导致鱼类淋巴细胞凋亡率更高（分别为单暴露组I（MC-LR）和II（LPS）的2.1倍和3.3倍），且存在显著的剂量反应关系。MC-LR与细菌LPS协同作用降低了谷胱甘肽S-转移酶（GST）的活性，增加了细胞内活性氧（ROS）和丙二醛（MDA）的水平，导致DNA损伤和细胞停滞于G0期，从而抑制细胞增殖并加速凋亡。证明了MC-LR通过与LPS协同作用加剧了鱼类免疫毒性，这对水产养殖业有严重的不利影响。
