多核苷酸激酶：功能纯化及其在通过限制性内切酶和其他作用有限的内切核酸酶对DNA单链和双链切割的直接动力学测量中的应用。 - Suppr

Suppr

超能文献

Polynucleotide kinase: functional purification and use in the direct kinetic measurement of single- and double- strand cleavages of DNA by restriction and other endonucleases of limited action.

作者信息

Kroeker W D, Laskowski M

出版信息

Anal Biochem. 1977 May 1;79(1-2):63-72. doi: 10.1016/0003-2697(77)90379-7.

DOI:10.1016/0003-2697(77)90379-7

PMID:194502

Abstract

摘要

相似文献

Polynucleotide kinase: functional purification and use in the direct kinetic measurement of single- and double- strand cleavages of DNA by restriction and other endonucleases of limited action.多核苷酸激酶：功能纯化及其在通过限制性内切酶和其他作用有限的内切核酸酶对DNA单链和双链切割的直接动力学测量中的应用。

Anal Biochem. 1977 May 1;79(1-2):63-72. doi: 10.1016/0003-2697(77)90379-7.

Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA.多核苷酸激酶交换反应：对DNA中限制性内切酶产生的5'-磷酸末端进行定量分析。

J Biol Chem. 1977 May 25;252(10):3176-84.

Partial characterization of an endonuclease activity which appears in nuclease free T4 polynucleotide kinase.在无核酸酶的T4多核苷酸激酶中出现的一种核酸内切酶活性的部分特性

Nucleic Acids Res. 1976 Nov;3(11):3133-41. doi: 10.1093/nar/3.11.3133.

Polynucleotide kinase exchange as an assay for class II restriction endonucleases.多核苷酸激酶交换作为II类限制性内切核酸酶的一种检测方法。

Methods Enzymol. 1980;65(1):28-36. doi: 10.1016/s0076-6879(80)65007-1.

Quantitation of the various termini generated by type II restriction endonucleases using the polynucleotide kinase exchange reaction.

J Biol Chem. 1979 Apr 10;254(7):2561-4.

5'-32P labeling of RNA and DNA restriction fragments.RNA和DNA限制性片段的5'-32P标记

Methods Enzymol. 1980;65(1):75-85. doi: 10.1016/s0076-6879(80)65012-5.

Enzymatic transfer of ATP gamma S thiophosphate onto the 5'-hydroxyl of an oligonucleotide as a route to reactive oligonucleotide derivatives.通过将三磷酸腺苷γ-硫代磷酸酯（ATPγS）的硫代磷酸基团酶促转移至寡核苷酸的5'-羟基上，来制备反应性寡核苷酸衍生物。

FEBS Lett. 1982 Jun 21;143(1):119-23. doi: 10.1016/0014-5793(82)80286-x.

The DNA 3'-phosphatase and 5'-hydroxyl kinase of rat liver chromatin.大鼠肝脏染色质的DNA 3'-磷酸酶和5'-羟基激酶

FEBS Lett. 1983 Aug 22;160(1-2):46-50. doi: 10.1016/0014-5793(83)80933-8.

Purification and properties of polynucleotide kinase of calf thymus.小牛胸腺多核苷酸激酶的纯化及性质

Biochim Biophys Acta. 1978 Feb 10;522(2):412-22. doi: 10.1016/0005-2744(78)90074-8.

3'-Phosphatase activity in T4 polynucleotide kinase.T4多核苷酸激酶中的3'-磷酸酶活性。

Biochemistry. 1977 Nov 15;16(23):5120-6. doi: 10.1021/bi00642a027.

引用本文的文献

Nucleotide sequence of the operators of lambda ultravirulent mutants.λ超毒力突变体操纵子的核苷酸序列。

Nucleic Acids Res. 1980 May 24;8(10):2147-64. doi: 10.1093/nar/8.10.2147.

Complete nucleotide sequence of minicircle kinetoplast DNA from Trypanosoma equiperdum.

Proc Natl Acad Sci U S A. 1981 Jun;78(6):3323-7. doi: 10.1073/pnas.78.6.3323.

Base specificity of mismatch repair in Streptococcus pneumoniae.肺炎链球菌错配修复的碱基特异性

Nucleic Acids Res. 1981 May 25;9(10):2267-80. doi: 10.1093/nar/9.10.2267.

Nucleotide sequence of the EcoRI D fragment of adenovirus 2 genome.腺病毒2型基因组EcoRI D片段的核苷酸序列

Nucleic Acids Res. 1980 May 24;8(10):2173-92. doi: 10.1093/nar/8.10.2173.

Localization on the viral genome and nucleotide sequence of the gene coding for the two major polypeptides of the hepatitis B surface antigen (HBs Ag).乙型肝炎表面抗原（HBs Ag）两种主要多肽编码基因在病毒基因组上的定位及核苷酸序列。

Nucleic Acids Res. 1979 Sep 25;7(2):335-46. doi: 10.1093/nar/7.2.335.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验