Yamashita A, Kawana A
NTT Basic Research Laboratories, Tokyo, Japan.
Neurosci Lett. 1991 Jul 22;128(2):147-9. doi: 10.1016/0304-3940(91)90248-r.
Nerve growth factor (NGF) rapidly infused into the extracellular medium near cell bodies of cultured chick dorsal root ganglion neurons caused transient increases in intracellular calcium ions, which was detected by fura-2 fluorometry. Similar increases were also observed in a calcium-free medium. There was typically a delay of 20 min between NGF infusion and the release of calcium ions, and this delay was longer when NGF was applied at neurite tips. This is probably because NGF must be transported from neurites to the cell body, where calcium ions are released from intracellular stores.
快速注入培养的鸡背根神经节神经元细胞体附近细胞外培养基中的神经生长因子（NGF），会导致细胞内钙离子短暂增加，这是通过fura-2荧光测定法检测到的。在无钙培养基中也观察到了类似的增加。NGF注入与钙离子释放之间通常有20分钟的延迟，当在神经突尖端应用NGF时，这种延迟会更长。这可能是因为NGF必须从神经突运输到细胞体，钙离子从细胞内储存库中释放出来的地方就在细胞体。
