Sernagor E, O'Donovan M J
Laboratory of Neural Control, National Institutes of Health, Bethesda, MD 20892.
Neurosci Lett. 1991 Jul 22;128(2):211-6. doi: 10.1016/0304-3940(91)90263-s.
Whole-cell patch clamp recordings were obtained during motor activity from electrically identified motoneurons within the spinal cord of the chick embryo maintained in vitro. Most recordings were performed on E11-E13 motoneurons although it was also possible to record from younger cells (E7-E9). Voltage clamp recordings were used to characterize the synaptic currents expressed in femoro-tibialis (extensor) motoneurons during motor activity. These motoneurons exhibited rhythmic excitatory currents with reversal potentials near 0 mV. This powerful technique enables high resolution recordings from identified motoneurons in situ and allows investigation of the membrane and synaptic mechanisms involved in the development of embryonic motility.
在体外培养的鸡胚脊髓内，对通过电刺激鉴定出的运动神经元进行运动活动期间的全细胞膜片钳记录。大多数记录是在E11 - E13运动神经元上进行的，不过也有可能从更年轻的细胞（E7 - E9）上进行记录。电压钳记录用于表征运动活动期间股胫（伸肌）运动神经元中表达的突触电流。这些运动神经元表现出节律性兴奋性电流，其反转电位接近0 mV。这项强大的技术能够对原位鉴定的运动神经元进行高分辨率记录，并允许研究胚胎运动发育中涉及的膜和突触机制。
