基于¹H NMR的羊水代谢组学用于胎儿畸形的代谢特征分析

(1)H NMR based metabonomics of human amniotic fluid for the metabolic characterization of fetus malformations.

作者信息

Graça Gonçalo, Duarte Iola F, Barros António S, Goodfellow Brian J, Diaz Sílvia, Carreira Isabel M, Couceiro Ana Bela, Galhano Eulália, Gil Ana M

机构信息

CICECO, Department of Chemistry, University of Aveiro, Portugal.

出版信息

J Proteome Res. 2009 Aug;8(8):4144-50. doi: 10.1021/pr900386f.

DOI:10.1021/pr900386f

PMID:19453159

Abstract

An NMR-metabonomic study of malformed fetuses was carried out through human amniotic fluid (HAF) analysis. Over 70 compounds were detected in control HAF by NMR. Possible confounding variables (fetus gender and gestational and maternal ages) were shown not to induce detectable compositional trends in the control group considered. Malformed fetuses showed variations in glucose, some amino acids and organic acids and proteins. In tandem with enzymatic assays, these NMR results suggest that changes in gycolysis and gluconeogenesis as well as kidney underdevelopment occur in the malformed fetuses studied here.

摘要

通过人羊水（HAF）分析对畸形胎儿进行了核磁共振代谢组学研究。通过核磁共振在对照HAF中检测到70多种化合物。结果表明，可能的混杂变量（胎儿性别、孕周和母亲年龄）在考虑的对照组中未引起可检测到的成分趋势变化。畸形胎儿在葡萄糖、一些氨基酸、有机酸和蛋白质方面表现出差异。结合酶活性测定，这些核磁共振结果表明，在此研究的畸形胎儿中发生了糖酵解和糖异生的变化以及肾脏发育不全。