de Ridder L, Mareel M, Vakaet L
Arch Geschwulstforsch. 1977;47(1):7-27.
To study malignant invasion, we associated tissue and cell culture fragments, with adhesive and non-adhesive living substrates in vitro (11). Non malignant mesonephros, quail heart and BHK-cells, as well as malignant HeLa-, Hepatoma-, Harding-Passey melanoma-, Py-, TLX5 lymphoma- and Schmidt-Ruppin sarcoma cells, were transplanted into cultured organ fragments of chick embryos and chick blastoderms. Malignant invasion was evaluated on the basis of the following histological criteria: 1. changes in organization of the graft. 2. infiltration of cells from the graft into the substrate, 3. degenerative alterations of the substrate. It was shown that adhesion of the graft to the substrate is a prerequisite for malignant invasion. Invasion into non-adhesive substrates, such as the apical side of epithelia, was never observed. Contrary, all malignant cells did invade into adhesive substrates. Interposition of a vitelline membrane always inhibited the expression of invasiveness. The morphological pattern of invasion depended mainly on the architecture of the substrate and differential resistance of its various components explained well all histological pictures. From the latter observation and from the localization of degenerative changes in the substrate we inferred that malignant cells exert their deleterious effect most probably upon immediate contact with their host.
为了研究恶性侵袭，我们在体外将组织和细胞培养片段与粘性和非粘性的活底物相结合（11）。将非恶性的中肾、鹌鹑心脏和BHK细胞，以及恶性的海拉细胞、肝癌细胞、哈丁 - 帕西黑色素瘤细胞、Py细胞、TLX5淋巴瘤细胞和施密特 - 鲁平肉瘤细胞移植到鸡胚和鸡胚盘的培养器官片段中。根据以下组织学标准评估恶性侵袭：1. 移植物组织结构的变化。2. 移植物中的细胞浸润到底物中。3. 底物的退行性改变。结果表明，移植物与底物的粘附是恶性侵袭的先决条件。从未观察到向非粘性底物（如上皮细胞的顶端侧）的侵袭。相反，所有恶性细胞都能侵入粘性底物。卵黄膜的插入总是会抑制侵袭性的表达。侵袭的形态模式主要取决于底物的结构，其各个成分的不同抗性很好地解释了所有组织学图像。从后一观察结果以及底物中退行性变化的定位，我们推断恶性细胞很可能在与宿主直接接触时发挥其有害作用。
