de Ridder L, Mareel M, Vakaet L
Cancer Res. 1975 Nov;35(11 Pt 1):3164-71.
To study adhesion, which is probably the initial step in malignant invasion, we associated tissue culture fragments with living substrates in vitro. Malignant HeLa, hepatoma, and PY cells, as well as nonmalignant BHK cells, were transplanted into cultured chick blastoderms and organ fragments from chick embryos. Adhesion was evaluated by time-lapse cinematography, by flushing with Tyrode's solution, and by histological examination after fixation. It was shown that the adhesion of these tissue culture fragments depends on the nature of the substrate. Substrates of connective tissue, mesenchyme, and the basal side of epithelia proved to be adhesive. In contrast, the apical side of intact epithelia was nonadhesive. Perforated epithelia allowed adhesion at the site of the perforation. In the presence of dilysine, HeLa cells adhere to the apical side of epithelia and to the dorsal side of the upper layer of the blastoderm. We concluded that the apical side of intact epithelia constitutes an inappropriate substrate for adhesion of a large variety of cells, in vitro as well as in vivo. Alteration of this characteristic in the presence of dilysine indicates that long-range electrostatic repulsion might be responsible for the nonadhesive character of the epithelia.
为了研究黏附（这可能是恶性侵袭的初始步骤），我们在体外将组织培养片段与活的底物相结合。将恶性的海拉细胞、肝癌细胞和PY细胞，以及非恶性的BHK细胞移植到培养的鸡胚胚盘和鸡胚器官片段中。通过延时摄影、用台氏液冲洗以及固定后的组织学检查来评估黏附情况。结果表明，这些组织培养片段的黏附取决于底物的性质。结缔组织、间充质以及上皮细胞的基底侧被证明具有黏附性。相反，完整上皮细胞的顶端侧不具有黏附性。有穿孔的上皮细胞在穿孔部位允许黏附。在存在双赖氨酸的情况下，海拉细胞会黏附到上皮细胞的顶端侧以及胚盘上层的背侧。我们得出结论，完整上皮细胞的顶端侧无论是在体外还是体内，对于多种细胞的黏附来说都是不合适的底物。在双赖氨酸存在的情况下这种特性的改变表明，长程静电排斥可能是上皮细胞非黏附特性的原因。
