用于检测血管内血栓的多模态纳米制剂。
Multimodal nanoagents for the detection of intravascular thrombi.
作者信息
McCarthy Jason R, Patel Purvish, Botnaru Ion, Haghayeghi Pouneh, Weissleder Ralph, Jaffer Farouc A
机构信息
Center for Molecular Imaging Research, Harvard Medical School, Massachusetts General Hospital, 149 13th Street, Room 5406, Charlestown, Massachusetts 02129, USA.
出版信息
Bioconjug Chem. 2009 Jun;20(6):1251-5. doi: 10.1021/bc9001163.
Abstract
Thrombosis underlies numerous life-threatening cardiovascular syndromes. Development of thrombosis-specific molecular imaging agents to detect and monitor thrombogenesis and fibrinolysis in vivo could improve the diagnosis, risk stratification, and treatment of thrombosis syndromes. To this end, we have synthesized efficient multimodal nanoagents targeted to two different constituents of thrombi, namely, fibrin and activated factor XIII. These agents are targeted via the conjugation of peptide-targeting ligands to the surface of fluorescently labeled magnetic nanoparticles. As demonstrated by in vitro and in vivo studies, both nanoagents possess high affinities for thrombi, and enable mutimodal fluorescence and magnetic resonance imaging.
摘要
血栓形成是众多危及生命的心血管综合征的基础。开发用于在体内检测和监测血栓形成及纤维蛋白溶解的血栓特异性分子成像剂，可改善血栓形成综合征的诊断、风险分层和治疗。为此，我们合成了靶向血栓两种不同成分（即纤维蛋白和活化因子 XIII）的高效多模态纳米剂。这些纳米剂通过将肽靶向配体与荧光标记的磁性纳米颗粒表面偶联来实现靶向。体外和体内研究表明，这两种纳米剂对血栓均具有高亲和力，并能实现多模态荧光和磁共振成像。
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本文引用的文献
1
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Circulation. 2008 Oct 28;118(18):1802-9. doi: 10.1161/CIRCULATIONAHA.108.785881. Epub 2008 Oct 13.
2
Molecular imaging of cardiovascular disease.心血管疾病的分子成像
Circulation. 2007 Aug 28;116(9):1052-61. doi: 10.1161/CIRCULATIONAHA.106.647164.
3
Localization and quantification of platelet-rich thrombi in large blood vessels with near-infrared fluorescence imaging.利用近红外荧光成像对大血管中富含血小板的血栓进行定位和定量分析。
Circulation. 2007 Jan 2;115(1):84-93. doi: 10.1161/CIRCULATIONAHA.106.643908. Epub 2006 Dec 18.
4
Imaging thromboembolism with fibrin-avid 99mTc-peptide: evaluation in swine.使用纤维蛋白亲和性99mTc肽成像血栓栓塞：在猪身上的评估
J Nucl Med. 2006 Jan;47(1):155-62.
5
Molecular imaging of factor XIIIa activity in thrombosis using a novel, near-infrared fluorescent contrast agent that covalently links to thrombi.使用一种与血栓共价连接的新型近红外荧光造影剂对血栓形成过程中因子ⅩⅢa活性进行分子成像。
Circulation. 2004 Jul 13;110(2):170-6. doi: 10.1161/01.CIR.0000134484.11052.44. Epub 2004 Jun 21.
6
Initial accumulation of platelets during arterial thrombus formation in vivo is inhibited by elevation of basal cAMP levels.体内动脉血栓形成过程中血小板的初始聚集受到基础环磷酸腺苷（cAMP）水平升高的抑制。
Blood. 2004 Mar 15;103(6):2127-34. doi: 10.1182/blood-2003-04-1133. Epub 2003 Nov 26.
7
Novel factor XIII probes for blood coagulation imaging.
Chembiochem. 2003 Sep 5;4(9):897-9. doi: 10.1002/cbic.200300602.
8
In vivo imaging of thrombin activity in experimental thrombi with thrombin-sensitive near-infrared molecular probe.使用凝血酶敏感型近红外分子探针在实验性血栓中对凝血酶活性进行体内成像。
Arterioscler Thromb Vasc Biol. 2002 Nov 1;22(11):1929-35. doi: 10.1161/01.atv.0000033089.56970.2d.
9
Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse.小鼠动脉血栓形成过程中血小板、组织因子和纤维蛋白的实时体内成像。
Nat Med. 2002 Oct;8(10):1175-81. doi: 10.1038/nm782. Epub 2002 Sep 16.
10
Fluorescence molecular tomography resolves protease activity in vivo.荧光分子断层成像可在体内解析蛋白酶活性。
Nat Med. 2002 Jul;8(7):757-60. doi: 10.1038/nm729. Epub 2002 Jun 24.
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