Matsumoto Shogo, Hull J Joe, Ohnishi Atsushi
Molecular Entomology Laboratory, RIKEN (The Institute of Physical and Chemical Research), Saitama, Japan.
Ann N Y Acad Sci. 2009 Apr;1163:464-8. doi: 10.1111/j.1749-6632.2008.03646.x.
Pheromone biosynthesis in the silkmoth Bombyx mori is under the control of the neurohormone pheromone biosynthesis activating neuropeptide (PBAN) and is triggered upon PBAN binding to its cognate PBAN receptor on the pheromone gland cells of female moths. Using fluorescent Ca(2+) imaging techniques with isolated pheromone glands, we have successfully demonstrated that PBAN specifically evokes an influx of extracellular Ca(2+). Furthermore, results from experiments designed to elucidate the molecular mechanisms underlying PBAN signaling indicate that B. mori utilizes the canonical store-operated channel-activation pathway and that the influx of extracellular Ca(2+) accelerates both lipolysis and fatty acyl reduction through a phosphorylation/dephosphorylation cascade for bombykol production.
家蚕的性信息素生物合成受神经激素性信息素生物合成激活肽（PBAN）的控制，当PBAN与雌蛾性信息素腺细胞上的同源PBAN受体结合时，该过程被触发。通过对分离出的性信息素腺使用荧光Ca(2+)成像技术，我们成功证明PBAN能特异性地引起细胞外Ca(2+)内流。此外，旨在阐明PBAN信号传导潜在分子机制的实验结果表明，家蚕利用经典的储存-操作性通道激活途径，细胞外Ca(2+)的内流通过磷酸化/去磷酸化级联反应加速脂肪分解和脂肪酰还原，从而产生蚕蛾醇。
