Hull J J, Lee J M, Matsumoto S
Molecular Entomology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan.
Insect Mol Biol. 2010 Aug;19(4):553-66. doi: 10.1111/j.1365-2583.2010.01013.x. Epub 2010 Jun 10.
Sex pheromone production for most moths is regulated by pheromone biosynthesis activating neuropeptide (PBAN). In Bombyx mori, PBAN binding triggers the opening of store-operated Ca(2+) channels, suggesting the involvement of a receptor-activated phospholipase C (PLC). In this study, we found that PLC inhibitors U73122 and compound 48/80 reduced sex pheromone production and that intracellular levels of (3)H-inositol phosphate species increased following PBAN stimulation. In addition, we amplified cDNAs from pheromone glands corresponding to PLCbeta1, PLCbeta4, PLCgamma and two G protein alpha subunits, Go and Gq. In vivo RNA interference-mediated knockdown analyses revealed that BmPLCbeta1, BmGq1, and unexpectedly, BmPLCgamma, are part of the PBAN signal transduction cascade.
大多数蛾类性信息素的产生受信息素生物合成激活神经肽（PBAN）调控。在家蚕中，PBAN结合会触发储存式钙离子通道开放，这表明存在受体激活的磷脂酶C（PLC）参与其中。在本研究中，我们发现PLC抑制剂U73122和化合物48/80可降低性信息素的产生，且PBAN刺激后细胞内（3）H-肌醇磷酸水平升高。此外，我们从信息素腺中扩增出了与PLCβ1、PLCβ4、PLCγ以及两个G蛋白α亚基Go和Gq相对应的cDNA。体内RNA干扰介导的敲低分析表明，BmPLCβ1、BmGq1以及出人意料的BmPLCγ是PBAN信号转导级联反应的一部分。
