Arthur Patrick K, Claussen Maike, Koch Susanne, Tarbashevich Katsiaryna, Jahn Olaf, Pieler Tomas
Department of Developmental Biochemistry, Göttingen Center for Molecular Biosciences, University of Göttingen, 37077 Göttingen, Germany.
J Biol Chem. 2009 Jul 24;284(30):19982-92. doi: 10.1074/jbc.M109.009928. Epub 2009 May 20.
Directional transport of specific mRNAs is of primary biological relevance. In Xenopus oocytes, mRNA localization to the vegetal pole is important for germ layer formation and germ cell development. Using a biochemical approach, we identified Xenopus Elr-type proteins, homologs of the Hu/ELAV proteins, as novel components of the vegetal mRNA localization machinery. They bind specifically to the localization elements of several different vegetally localizing Xenopus mRNAs, and they are part of one RNP together with other localization proteins, such as Vg1RBP and XStaufen 1. Blocking Elr-type protein binding by either localization element mutagenesis or antisense morpholino oligonucleotide-mediated masking of their target RNA structures, as well as overexpression of wild type and mutant ElrB proteins, interferes with vegetal localization in Xenopus oocytes.
特定mRNA的定向运输具有重要的生物学意义。在非洲爪蟾卵母细胞中，mRNA定位于植物极对于胚层形成和生殖细胞发育至关重要。我们采用生化方法，鉴定出非洲爪蟾Elr型蛋白（Hu/ELAV蛋白的同源物）是植物mRNA定位机制的新组分。它们特异性结合几种不同的定位于植物极的非洲爪蟾mRNA的定位元件，并且与其他定位蛋白（如Vg1RBP和XStaufen 1）一起构成一个核糖核蛋白颗粒。通过定位元件诱变或反义吗啉代寡核苷酸介导的对其靶RNA结构的掩蔽来阻断Elr型蛋白的结合，以及野生型和突变型ElrB蛋白的过表达，均会干扰非洲爪蟾卵母细胞中的植物极定位。
