Institute of Physiology and Pathophysiology, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, 55128 Mainz, Germany.
EMBO Rep. 2012 Sep;13(9):827-34. doi: 10.1038/embor.2012.97. Epub 2012 Jun 29.
Oligodendroglial Myelin Basic Protein (MBP) synthesis is essential for myelin formation in the central nervous system. During oligodendrocyte differentiation, MBP mRNA is kept in a translationally silenced state while intracellularly transported, until neuron-derived signals initiate localized MBP translation. Here we identify the small non-coding RNA 715 (sncRNA715) as an inhibitor of MBP translation. SncRNA715 localizes to cytoplasmic granular structures and associates with MBP mRNA transport granule components. We also detect increased levels of sncRNA715 in demyelinated chronic human multiple sclerosis lesions, which contain MBP mRNA but lack MBP protein.
少突胶质细胞髓鞘碱性蛋白(MBP)的合成对于中枢神经系统髓鞘的形成是必不可少的。在少突胶质细胞分化过程中,MBP mRNA 保持在翻译沉默状态,同时在细胞内运输,直到神经元衍生的信号启动局部 MBP 翻译。在这里,我们发现小非编码 RNA 715(sncRNA715)是 MBP 翻译的抑制剂。sncRNA715 定位于细胞质颗粒状结构,并与 MBP mRNA 运输颗粒成分结合。我们还在脱髓鞘的慢性人类多发性硬化病变中检测到 sncRNA715 水平升高,这些病变包含 MBP mRNA,但缺乏 MBP 蛋白。