Wang Xiaoke, Liang Likun, Song Tao, Wu Longfei
Weifang Medical University, Weifang, 261042, China.
Curr Microbiol. 2009 Sep;59(3):221-6. doi: 10.1007/s00284-009-9418-7. Epub 2009 May 21.
To investigate the effects of pulsed magnetic field on magnetosome formation in Magnetospirillum magneticum AMB-1, cultures inoculated with either mangetic or non-magnetic pre-cultures were incubated under 1 mT pulsed magnetic field. Magnetism of cells was measured by using spectrophotometer coupled with applied magnetic fields and the values were described as C(mag). Magnetosome in cells was counted by transmission electron microscopy observation. The results showed that pulsed magnetic field did not affect cellular growth, but enhanced magnetosome formation. The applied pulsed magnetic field might exceed the chain of magnetosomes and change the homogeneity of the magnetosome particles. The results implied that magnetite precipitation induced by the adjacent magnetosome was affected by pulsed magnetic field. Moreover, the applied pulsed magnetic field up-regulated the magA and mamA expression in cells, which might account for the increasing number and the exceeding chain of magnetosomes in cells.
为研究脉冲磁场对趋磁螺菌AMB-1中磁小体形成的影响，将接种磁性或非磁性预培养物的培养物在1 mT脉冲磁场下孵育。通过使用与施加磁场耦合的分光光度计测量细胞的磁性，其值记为C(mag)。通过透射电子显微镜观察对细胞中的磁小体进行计数。结果表明，脉冲磁场不影响细胞生长，但增强了磁小体的形成。施加的脉冲磁场可能超过磁小体链并改变磁小体颗粒的均匀性。结果表明，相邻磁小体诱导的磁铁矿沉淀受脉冲磁场影响。此外，施加的脉冲磁场上调了细胞中magA和mamA的表达，这可能解释了细胞中磁小体数量增加和磁小体链超过正常的原因。
