Całka J, Franke-Radowiecka A, Załecki M, Łakomy M
University of Warmia and Mazury, Faculty of Veterinary Medicine, Department of Clinical Physiology, Oczapowskiego 13, 10-719 Olsztyn, Poland.
Pol J Vet Sci. 2009;12(1):61-7.
Previous immunocytochemical studies provided conflicting data concerning occurrence of the CGRP-immunoreactive neuronal cell bodies in the porcine spinal cord. In the present study, we have investigated expression of the CGRP and its possible coexpression with ChAT in the gray matter of the thoracic, lumbar and sacral spinal cord of the pig. Our study revealed a large number of CGRP-immunoreactive cells in the motor nucleus of the ventral horn, and less and singe perikarya intermediolateral and intermediomedial nuclei, respectively. Double staining immunocytochemistry, depending on the cross-section level, disclosed the highest ChAT/CGRP colocalization subsequently in the motor nucleus of the ventral horn, then in the intermediolateral and intermediomedial nucleus. Our data provide morphological evidence confirming expression of CGRP in the porcine spinal nuclei while its coexpression in cholinergic neurons suggests that CGRP may play a role in modulation of the spinal cholinergic transmission.
以往的免疫细胞化学研究关于猪脊髓中降钙素基因相关肽（CGRP）免疫反应性神经元细胞体的出现提供了相互矛盾的数据。在本研究中，我们调查了猪胸段、腰段和骶段脊髓灰质中CGRP的表达及其与胆碱乙酰转移酶（ChAT）的可能共表达。我们的研究显示，腹角运动核中有大量CGRP免疫反应性细胞，而中间外侧核和中间内侧核中分别较少且单个存在核周体。双重染色免疫细胞化学根据横断面水平显示，随后在腹角运动核中ChAT/CGRP共定位最高，然后在中间外侧核和中间内侧核中。我们的数据提供了形态学证据，证实了CGRP在猪脊髓核中的表达，而其在胆碱能神经元中的共表达表明CGRP可能在脊髓胆碱能传递的调节中起作用。
